High-Performance Liquid Chromatographic Method for The Separation of Enantiomeric Gatifloxacin
| Autor: | Srinivasulu Kota, Bujjibabu Lingavarapu, Koteshwara Mudigonda, Hima Bindu Vurimindi, Vishwottam Kandikere, Ramakrishna Nirogi, Saritha Vennila |
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| Rok vydání: | 2010 |
| Předmět: |
Resolution (mass spectrometry)
Phenylcarbamates Analytical chemistry Gatifloxacin High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Drug Stability Limit of Detection Amylose Phase (matter) medicine Chromatography High Pressure Liquid Antibacterial agent Detection limit Chromatography Chemistry Stereoisomerism General Medicine Anti-Bacterial Agents Calibration Enantiomer Fluoroquinolones medicine.drug |
| Zdroj: | Journal of Chromatographic Science. 48:100-103 |
| ISSN: | 1945-239X 0021-9665 |
| DOI: | 10.1093/chromsci/48.2.100 |
| Popis: | A high-performance liquid chromatographic method has been developed in normal-phase conditions for the separation of enantiomeric gatifloxacin, (+/-) 1-cyclopropyl-6-fluoro-8-methoxy-7-(3-methylpiperazin-1-yl)-4-oxo-quinoline-3-carboxylic acid, an antibiotic in bulk drug. The method involved the use of an amylose-based Chiralpak AD-H (150 mm x 4.6 mm, 5 microm) column using a mobile phase system containing n-hexane-ethanol-diethylamine (85:15:0.1% v/v). The conditions affording the best resolution were found by selection and variation of the mobile-phase compositions and the differences in separation capability of the method is noted. Relative standard deviation of retention times and peak areas were better than 0.2% and 0.4%, respectively, for precision. Gatifloxacin sample solution and mobile phase are found to be stable for at least 48 h. |
| Databáze: | OpenAIRE |
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