MicroRNA-25 functions as a potential tumor suppressor in colon cancer by targeting Smad7
| Autor: | Ji Tao, Chunjing Zhang, Chaoxia Zou, Zhongjing Han, Xishan Wang, Li Qiang, Xu Gao, Lei Zhang, Chendan Zou, Qingchao Tang, Huiyan Wei, Wei Wang, Haifeng Xiao, Xueying Zhang |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
Oncology Cancer Research medicine.medical_specialty Colorectal cancer Down-Regulation Mice Nude Biology Smad7 Protein law.invention Mice Downregulation and upregulation Cell Movement In vivo RNA interference law Internal medicine microRNA medicine Animals Humans Genes Tumor Suppressor 3' Untranslated Regions Aged Regulation of gene expression Mice Inbred BALB C Binding Sites Base Sequence Cell growth Middle Aged HCT116 Cells medicine.disease Tumor Burden Gene Expression Regulation Neoplastic MicroRNAs Colonic Neoplasms Cancer research Suppressor Female RNA Interference Neoplasm Transplantation |
| Zdroj: | Cancer Letters. 335:168-174 |
| ISSN: | 0304-3835 |
| DOI: | 10.1016/j.canlet.2013.02.029 |
| Popis: | Because it is a member of the miR-106b~25 cluster, microRNA-25 (miR-25) is known to be dysregulated in human cancers. However, the expression and role of miR-25 in colon cancer remain unclear. In this study, miR-25 was found to be down-regulated in human colon cancer tissues when compared to those in matched, non-neoplastic mucosa tissues. Functional studies revealed that restoration of miR-25 expression inhibited cell proliferation and migration. In contrast, miR-25 inhibition could promote the proliferation and migratory ability of cells. Stable over-expression of miR-25 also suppressed the growth of colon cancer-cell xenografts in vivo. Furthermore, bioinformatic predictions and experimental validation were used to identify Smad7 as a direct target of miR-25. Functional reverse experiments indicated that the antitumor effects of miR-25 were probably mediated by its repression of Smad7. These results suggest that miR-25 may function as a tumor suppressor by targeting Smad7 in colon cancer. Thus, miR-25 may serve as a potential therapeutic agent or target for cancer therapy. |
| Databáze: | OpenAIRE |
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