A unique miR775-GALT9 module regulates leaf senescence in Arabidopsis during post-submergence recovery by modulating ethylene and the abscisic acid pathway
| Autor: | Vishnu Mishra, Archita Singh, Nidhi Gandhi, Shabari Sarkar Das, Sandeep Yadav, Ashutosh Kumar, Ananda K. Sarkar |
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| Rok vydání: | 2022 |
| Předmět: |
Binding Sites
Arabidopsis Proteins Arabidopsis Golgi Apparatus NADPH Oxidases Receptors Cell Surface Ethylenes Galactosyltransferases Plants Genetically Modified Dioxygenases Plant Senescence Plant Leaves Cysteine Endopeptidases MicroRNAs Stress Physiological Reactive Oxygen Species Molecular Biology Abscisic Acid Plant Proteins Signal Transduction Developmental Biology |
| Zdroj: | Development. 149 |
| ISSN: | 1477-9129 0950-1991 |
| Popis: | The submergence-induced hypoxic condition negatively affects the plant growth and development, and causes early onset of senescence. Hypoxia alters the expression of a number of microRNAs (miRNAs). However, the molecular function of submergence stress-induced miRNAs in physiological or developmental changes and recovery remains poorly understood. Here, we show that miR775 is an Arabidopsis thaliana-specific young and unique miRNA that possibly evolved non-canonically. miR775 post-transcriptionally regulates GALACTOSYLTRANSFERASE 9 (GALT9) and their expression is inversely affected at 24 h of complete submergence stress. The overexpression of miR775 (miR775-Oe) confers enhanced recovery from submergence stress and reduced accumulation of RBOHD and ROS, in contrast to wild-type and MIM775 Arabidopsis shoot. A similar recovery phenotype in the galt9 mutant indicates the role of the miR775-GALT9 module in post-submergence recovery. We predicted that Golgi-localized GALT9 is potentially involved in protein glycosylation. The altered expression of senescence-associated genes (SAG12, SAG29 and ORE1), ethylene signalling (EIN2 and EIN3) and abscisic acid (ABA) biosynthesis (NCED3) pathway genes occurs in miR775-Oe, galt9 and MIM775 plants. Thus, our results indicate the role for the miR775-GALT9 module in post-submergence recovery through a crosstalk between the ethylene signalling and ABA biosynthesis pathways. |
| Databáze: | OpenAIRE |
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