β-Carotene inhibition of 7,12-dimethylbenz[a]anthracene-induced transformation of murine mammary cells in vitro
| Autor: | Mihir R. Banerjee, Malay Chatterjee, Subhendu Som |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Vitamin
Cancer Research medicine.medical_specialty 9 10-Dimethyl-1 2-benzanthracene medicine.medical_treatment Mammary gland Sister chromatid exchange In Vitro Techniques Biology Epithelium Mice chemistry.chemical_compound Mammary Glands Animal Internal medicine medicine Animals Mice Inbred BALB C 7 12-Dimethylbenz[a]anthracene Carotene Retinol General Medicine beta Carotene Carotenoids In vitro Cell Transformation Neoplastic Endocrinology medicine.anatomical_structure chemistry Female Hormone |
| Zdroj: | Carcinogenesis. 5:937-940 |
| ISSN: | 1460-2180 0143-3334 |
| DOI: | 10.1093/carcin/5.7.937 |
| Popis: | The chemopreventive action of beta-carotene during chemically-induced transformation of the epithelial cells in organ culture of the whole mammary glands from BALB/c female mice was studied. The mammary epithelial cells in the whole mammary organ in a hormone supplemented, serum-free medium were transformed after 24 h exposure to 7.8 microM 7,12-dimethylbenz[a]anthracene (DMBA) between 3rd and 4th day of a total 10 day culture period. The transformation process was associated with appearance of nodule-like alveolar lesions (NLAL) in glands in vitro. The epithelial cells transformed by DMBA are potentially neoplastic, and NLAL serves as a morphological marker of preneoplasia in the glands in vitro. Treatment with beta-carotene (10(-6) M) during DMBA exposure (3rd-4th day) caused 68% inhibition in the number of glands with incidence of NLAL. A 49% inhibition of NLAL was evident when the glands were incubated with beta-carotene (days 4-10) after exposure to DMBA. Results indicate that beta-carotene inhibits DMBA-induced transformation of the mammary glands in vitro acting both at the initiation and the promotional stages. This inhibitory effect is likely due to the action of beta-carotene itself since no accumulation of retinol, the metabolic derivative of the vitamin A precursor, was detectable in the mammary glands during the 10 day culture period. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|