Promoter-Proximal Introns in Arabidopsis thaliana Are Enriched in Dispersed Signals that Elevate Gene Expression
| Autor: | Tali Elfersi, Alan B. Rose, Ian F Korf, Genís Parra |
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| Rok vydání: | 2008 |
| Předmět: |
Genetics
Messenger RNA Oryza sativa Arabidopsis Intron food and beverages Oryza Cell Biology Plant Science Group II intron Biology biology.organism_classification Introns Gene Expression Regulation Plant Intron-mediated enhancement Gene expression Group I catalytic intron Promoter Regions Genetic Research Articles |
| Zdroj: | The Plant Cell. 20:543-551 |
| ISSN: | 1532-298X |
| Popis: | Introns that elevate mRNA accumulation have been found in a wide range of eukaryotes. However, not all introns affect gene expression, and direct testing is currently the only way to identify stimulatory introns. Our genome-wide analysis in Arabidopsis thaliana revealed that promoter-proximal introns as a group are compositionally distinct from distal introns and that the degree to which an individual intron matches the promoter-proximal intron profile is a strong predictor of its ability to increase expression. We found that the sequences responsible for elevating expression are dispersed throughout an enhancing intron, as is a candidate motif that is overrepresented in first introns and whose occurrence in tested introns is proportional to its effect on expression. The signals responsible for intron-mediated enhancement are apparently conserved between Arabidopsis and rice (Oryza sativa) despite the large evolutionary distance separating these plants. |
| Databáze: | OpenAIRE |
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