Spermatozoa and spermatids retrieved from frozen reproductive organs or frozen whole bodies of male mice can produce normal offspring
| Autor: | Kazuto Morozumi, Atsuo Ogura, Keiji Mochida, Takamasa Iwaki, Yuichi Obata, Kazuo Moriwaki, Ryuzo Yanagimachi, Hiromi Miki, Kimiko Inoue, Martin Fray, Narumi Ogonuki |
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| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Male
endocrine system Time Factors Offspring media_common.quotation_subject Mammalian embryology Male mice Fertilization in Vitro Biology Cryopreservation Specimen Handling Andrology Mice Freezing Testis medicine Animals media_common Epididymis Mice Inbred BALB C Multidisciplinary Reproduction Temperature Embryo Biological Sciences Embryo Mammalian Sperm Spermatids Mice Inbred C57BL medicine.anatomical_structure Immunology Oocytes Female |
| Popis: | Cryopreservation of male germ cells is a strategy to conserve animal species and strains of animals valuable to biomedical research. We tested whether mouse male germ cells could be cryopreserved without cryoprotection by simply freezing epididymides, testes, or whole bodies. The reproductive organs were isolated from killed mice and frozen for 1 week to 1 year at −80°C before spermatozoa and spermatids were collected and injected into mature oocytes. Normal pups were born irrespective of strains tested (ICR and C57BL/6). Epididymides and testes frozen and transported internationally to another laboratory by air could produce pups of inbred C57BL/6 mice. Testicular spermatozoa retrieved from the bodies of male mice (BALB/c nude and C3H/He strains) that had been kept frozen (−20°C) for 15 years could also produce normal offspring by microinsemination. Thus, freezing of either male reproductive organs or whole bodies is the simplest way to preserve male germ cells. Restoration of extinct species could be possible if male individuals are found in permafrost. |
| Databáze: | OpenAIRE |
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