Fermentation and genomic analysis of acetone-uncoupled butanol production by Clostridium tetanomorphum
| Autor: | Hongjun Dong, Yanping Zhang, Guanhui Bao, Chunhua Zhao, Fuyu Gong, Yin Li |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Stereochemistry Molecular Sequence Data Butyrate Applied Microbiology and Biotechnology Acetone 03 medical and health sciences chemistry.chemical_compound 1-Butanol Bacterial Proteins Transferase Ethanol Strain (chemistry) organic chemicals Butanol General Medicine Genomics equipment and supplies Clostridium tetanomorphum carbohydrates (lipids) 030104 developmental biology chemistry Biochemistry Yield (chemistry) Fermentation bacteria lipids (amino acids peptides and proteins) Genome Bacterial Biotechnology |
| Zdroj: | Applied microbiology and biotechnology. 100(3) |
| ISSN: | 1432-0614 0000-0000 |
| Popis: | In typical acetone-butanol-ethanol (ABE) fermentation, acetone is the main by-product (50 % of butanol mass) of butanol production, resulting in a low yield of butanol. It is known that some Clostridium tetanomorphum strains are able to produce butanol without acetone in nature. Here, we described that C. tetanomorphum strain DSM665 can produce 4.16 g/L butanol and 4.98 g/L ethanol at pH 6.0, and 9.81 g/L butanol and 1.01 g/L ethanol when adding 1 mM methyl viologen. Butyrate and acetate could be reassimilated and no acetone was produced. Further analysis indicated that the activity of the acetate/butyrate:acetoacetyl-CoA transferase responsible for acetone production is lost in C. tetanomorphum DSM665. The genome of C. tetanomorphum DSM665 was sequenced and deposited in DDBJ, EMBL, and GenBank under the accession no. APJS00000000. Sequence analysis indicated that there are no typical genes (ctfA/B and adc) that are typically parts of an acetone synthesis pathway in C. tetanomorphum DSM665. This work provides new insights in the mechanism of clostridial butanol production and should prove useful for the design of a high-butanol-producing strain. |
| Databáze: | OpenAIRE |
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