Narrow Leaf21, encoding ribosomal protein RPS3A, controls leaf development in rice
| Autor: | Jingjing Fang, Xueyong Li, Haixin Long, Jinfeng Zhao, Syed Adeel Zafar, Shoujiang Yuan, Suyash B. Patil, Lixiang Peng, Lu Li, Yan Chun, Muhammad Uzair |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Ribosomal Proteins
0106 biological sciences Untranslated region Regular Issue Physiology Mutant Plant Science Biology 01 natural sciences Ribosome 03 medical and health sciences Ribosomal protein Translational regulation Genetics Eukaryotic Small Ribosomal Subunit Ribosome profiling Plant Proteins 030304 developmental biology 0303 health sciences fungi food and beverages Oryza Translation (biology) Cell biology Plant Leaves 010606 plant biology & botany |
| Zdroj: | Plant Physiol |
| ISSN: | 1532-2548 0032-0889 |
| DOI: | 10.1093/plphys/kiab075 |
| Popis: | Leaf morphology influences photosynthesis, transpiration, and ultimately crop yield. However, the molecular mechanism of leaf development is still not fully understood. Here, we identified and characterized the narrow leaf21 (nal21) mutant in rice (Oryza sativa), showing a significant reduction in leaf width, leaf length and plant height, and increased tiller number. Microscopic observation revealed defects in the vascular system and reduced epidermal cell size and number in the nal21 leaf blade. Map-based cloning revealed that NAL21 encodes a ribosomal small subunit protein RPS3A. Ribosome-targeting antibiotics resistance assay and ribosome profiling showed a significant reduction in the free 40S ribosome subunit in the nal21 mutant. The nal21 mutant showed aberrant auxin responses in which multiple auxin response factors (ARFs) harboring upstream open-reading frames (uORFs) in their 5′-untranslated region were repressed at the translational level. The WUSCHEL-related homeobox 3A (OsWOX3A) gene, a key transcription factor involved in leaf blade lateral outgrowth, is also under the translational regulation by RPS3A. Transformation with modified OsARF11, OsARF16, and OsWOX3A genomic DNA (gDNA) lacking uORFs rescued the narrow leaf phenotype of nal21 to a better extent than transformation with their native gDNA, implying that RPS3A could regulate translation of ARFs and WOX3A through uORFs. Our results demonstrate that proper translational regulation of key factors involved in leaf development is essential to maintain normal leaf morphology. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|