Detection and Identification of Protein Citrullination in Complex Biological Systems
| Autor: | Eranthie Weerapana, Paul R. Thompson, Kathleen W. Clancy |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Arginine Biochemistry Protein citrullination Article Mass Spectrometry Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Antigen Citrulline medicine Animals Humans Systemic lupus erythematosus Autoantibody Protein-arginine deiminase Citrullination Proteins medicine.disease 030104 developmental biology chemistry Rheumatoid arthritis Protein Processing Post-Translational |
| Popis: | Protein citrullination is a post-translational modification of arginine that is catalyzed by the Protein Arginine Deiminase (PAD) family of enzymes. Aberrantly increased citrullination is associated with a host of inflammatory diseases and cancer and PAD inhibitors have shown remarkable efficacy in a range of diseases including rheumatoid arthritis, lupus, atherosclerosis, and ulcerative colitis. In rheumatoid arthritis, citrullinated proteins serve as key antigens for rheumatoid arthritis-associated autoantibodies. These data suggest that citrullinated proteins may serve more generally as biomarkers of specific disease states, however, the identification of citrullinated residues remains challenging due to the small 1 Da mass change that occurs upon citrullination. Herein, we highlight the available techniques to identify citrullinated proteins/residues focusing on advanced MS techniques as well as chemical derivatization strategies that are currently being employed to identify citrullinated proteins as well as the specific residues modified within those proteins. |
| Databáze: | OpenAIRE |
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