Human amniotic epithelial cells as feeder layer to derive and maintain human embryonic stem cells from poor-quality embryos
| Autor: | Héctor Flores-Herrera, Elsa Romelia Moreno-Verduzco, Eva Vega-Hernández, Guadalupe García-López, Guadalupe Razo-Aguilera, Daniela Ávila-González, Néstor F. Díaz, Néstor E. Díaz-Martínez, Wendy Portillo, Anayansi Molina-Hernández, Julio Francisco De la Jara-Díaz, Irma Lydia García-Castro, Juan Carlos Regalado-Hernández |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
KOSR
Cellular differentiation Human Embryonic Stem Cells Biology Embryo Culture Techniques Feeder Layer Humans Amnion lcsh:QH301-705.5 Cells Cultured Genetics Medicine(all) Feeder Cells Cell Differentiation Epithelial Cells Amniotic stem cells General Medicine Cell Biology Embryo Mammalian Embryonic stem cell Cell biology lcsh:Biology (General) Karyotyping Amniotic epithelial cells embryonic structures Stem cell Transcription Factors Adult stem cell Developmental Biology |
| Zdroj: | Stem Cell Research, Vol 15, Iss 2, Pp 322-324 (2015) |
| ISSN: | 1873-5061 |
| DOI: | 10.1016/j.scr.2015.07.006 |
| Popis: | Data from the literature suggest that human embryonic stem cell (hESC) lines used in research do not genetically represent all human populations. The derivation of hESC through conventional methods involve the destruction of viable human embryos, as well the use of mouse embryonic fibroblasts as a feeder layer, which has several drawbacks. We obtained the hESC line (Amicqui-1) from poor-quality (PQ) embryos derived and maintained on human amniotic epithelial cells (hAEC). This line displays a battery of markers of pluripotency and we demonstrated the capacity of these cells to produce derivates of the three germ layers. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|