Assessment of Genetic Fidelity of in vitro Raised Plants in Swertia chirayita through ISSR, RAPD analysis and Peroxidase Profiling during Organogenesis
| Autor: | Nidhi S. Belwal, Barkha Kamal, A. K. Dobriyal, Vikash S. Jadon, Vikas Sharma |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine food.ingredient micropropagation lcsh:Biotechnology Organogenesis Biology 01 natural sciences Butyric acid 03 medical and health sciences chemistry.chemical_compound food Auxin RAPD lcsh:TP248.13-248.65 Botany ascorbate peroxidase chemistry.chemical_classification Multidisciplinary fungi ISSR food and beverages Swertia chirayita 030104 developmental biology adenine sulfate chemistry Micropropagation Herb Shoot biology.protein 010606 plant biology & botany Peroxidase |
| Zdroj: | Brazilian Archives of Biology and Technology v.59 2016 Brazilian Archives of Biology and Technology Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR Brazilian Archives of Biology and Technology, Vol 59, Iss 0 Brazilian Archives of Biology and Technology, Volume: 59, Article number: e16160389, Published: 28 NOV 2016 |
| Popis: | An efficient in vitro regeneration protocol for medicinally important herb Swertia chirayita was developed and the genetic fidelity was assessed using RAPD and ISSR markers. The best shoot regeneration was observed on MS basal supplemented with 1.0 mg/L Benzyl amino purine (BAP) in combination with Indole-3-acetic acid (IAA) (0.5 mg/L) that resulted in the increase by multiplication rate (7.65) with an average of 33.33 numbers of shoots and average shoot length of 2.70 cm. It was further enhanced by the addition of adenine sulfate (0.007%) that resulted in an average of 42 shoots per clum with 4.13 cm of average shoot length and the increase in multiplication fold to 9.75 that further resulted in the reduced use of other cytokinins and auxins. The rooting was nearly 100 % on 1/4 MS augmented with 1.0 mg/L Indole butyric acid with maximum average root length of 5.1cm. Plantlets were successfully acclimatized with 85-90 % survival rate. Ascorbate peroxidase activity increased with the maximum activity during the shoot multiplication. Clonal fidelity has been checked by two marker systems ISSR and RAPD and regenerated plants showed high clonal fidelity. |
| Databáze: | OpenAIRE |
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