Gene Expression Profiling of Mouse Condylar Cartilage during Mastication by Means of Laser Microdissection and cDNA Array
| Autor: | Junichi Watahiki, Tetsutaro Yamaguchi, Haruhisa Nakano, Tetsuhiko Tachikawa, Kotaro Maki, Tarou Irie |
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| Rok vydání: | 2004 |
| Předmět: |
Male
0301 basic medicine Microarray Sialoglycoproteins Biology Mice Transforming Growth Factor beta2 03 medical and health sciences 0302 clinical medicine stomatognathic system Insulin-Like Growth Factor II Transforming Growth Factor beta Complementary DNA Gene expression medicine Animals Hedgehog Proteins Lectins C-Type Aggrecans Growth Plate Insulin-Like Growth Factor I General Dentistry Mastication Heat-Shock Proteins Aggrecan Oligonucleotide Array Sequence Analysis Laser capture microdissection Extracellular Matrix Proteins Mice Inbred ICR Gene Expression Profiling Cartilage Mandibular Condyle Parathyroid Hormone-Related Protein 030206 dentistry Phosphoproteins Molecular biology Gene expression profiling 030104 developmental biology medicine.anatomical_structure Chondroitin Sulfate Proteoglycans Trans-Activators Osteopontin Proteoglycans Laser Therapy Microdissection |
| Zdroj: | Journal of Dental Research. 83:245-249 |
| ISSN: | 1544-0591 0022-0345 |
| DOI: | 10.1177/154405910408300312 |
| Popis: | Little is known about the mechanisms of mandibular condylar growth. In this study, gene expression in the mandibular condylar cartilage of young post-natal mice was monitored by means of a cDNA microarray, real-time PCR, and laser microdissection before and after the initiation of mastication (newborn, 7 days, 21 days, initiation of mastication, and 35 days). Insulin-like growth factor-1 (IGF-I), transforming-growth-factor-beta-2 (TGFbeta2), and aggrecan mRNAs were clearly expressed at 21 days, while the expression of osteopontin mRNAs was most clear at 35 days. Parathyroid-hormone-related protein (PTHrP), Indian-hedgehog (Ihh), and insulin-like growth factor-2 (IGF-2) mRNAs were clearly expressed during lactation (newborn and 7 days). Heat-shock-protein 84 (HSP-84) and heat-shock-protein 86 (HSP-86) were clearly expressed at 35 days. These results revealed that gene expression changed during mandibular condylar cartilage growth, and that, interestingly, these changes coincided with the initiation of mastication. |
| Databáze: | OpenAIRE |
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