Enhanced bone formation in sheep vertebral bodies after minimally invasive treatment with a novel, PLGA fiber-reinforced brushite cement
| Autor: | Jens Günster, Klaus D. Jandt, Matthias Bungartz, Sabine Bischoff, Olaf Brinkmann, Victoria Horbert, Andre Sachse, Long Xin, Raimund W. Kinne, Harald Schubert, Stefan Maenz, Francesca Gunnella, Elke Kunisch, Bernhard Illerhaus, Jörg Bossert |
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| Rok vydání: | 2017 |
| Předmět: |
Calcium Phosphates
medicine.medical_specialty Bone Regeneration 0206 medical engineering Context (language use) macromolecular substances 02 engineering and technology chemistry.chemical_compound Polylactic Acid-Polyglycolic Acid Copolymer Osteogenesis In vivo Animals Medicine Orthopedics and Sports Medicine Brushite Lactic Acid Fiber Bone regeneration Bone mineral Vertebroplasty Sheep business.industry Osteoid Bone Cements technology industry and agriculture 021001 nanoscience & nanotechnology 020601 biomedical engineering Surgery PLGA chemistry Female Neurology (clinical) 0210 nano-technology business Polyglycolic Acid Biomedical engineering |
| Zdroj: | The Spine Journal. 17:709-719 |
| ISSN: | 1529-9430 |
| DOI: | 10.1016/j.spinee.2016.11.006 |
| Popis: | Background Context Injectable, brushite-forming calcium phosphate cements (CPC) show potential for bone replacement, but they exhibit low mechanical strength. This study tested a CPC reinforced with poly(l-lactide-co-glycolide) acid (PLGA) fibers in a minimally invasive, sheep lumbar vertebroplasty model. Purpose The study aimed to test the in vivo biocompatibility and osteogenic potential of a PLGA fiber-reinforced, brushite-forming CPC in a sheep large animal model. Study Design/Setting This is a prospective experimental animal study. Methods Bone defects (diameter: 5 mm) were placed in aged, osteopenic female sheep, and left empty (L2) or injected with pure CPC (L3) or PLGA fiber-reinforced CPC (L4; fiber diameter: 25 µm; length: 1 mm; 10% [wt/wt]). Three and 9 months postoperation (n=20 each), the structural and functional CPC effects on bone regeneration were documented ex vivo by osteodensitometry, histomorphometry, micro-computed tomography (micro-CT), and biomechanical testing. Results Addition of PLGA fibers enhanced CPC osteoconductivity and augmented bone formation. This was demonstrated by (1) significantly enhanced structural (bone volume/total volume, shown by micro-CT and histomorphometry; 3 or 9 months) and bone formation parameters (osteoid volume and osteoid surface; 9 months); (2) numerically enhanced bone mineral density (3 and 9 months) and biomechanical compression strength (9 months); and (3) numerically decreased bone erosion (eroded surface; 3 and 9 months). Conclusions The PLGA fiber-reinforced CPC is highly biocompatible and its PLGA fiber component enhanced bone formation. Also, PLGA fibers improve the mechanical properties of brittle CPC, with potential applicability in load-bearing areas. |
| Databáze: | OpenAIRE |
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