Postmortem analysis of famprofazone and its metabolites, methamphetamine and amphetamine, in porcine bone marrow
| Autor: | Silvana Vianna Rodrigues, Antonio Assis Vieira, Eliani Spinelli, Elisangela de S. Santos |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Swine
02 engineering and technology Urine 01 natural sciences Analytical Chemistry Methamphetamine Bone Marrow Porcine bone medicine Animals Famprofazone Pyrazolones Amphetamine Chromatography Chemistry 010401 analytical chemistry 021001 nanoscience & nanotechnology 0104 chemical sciences Time of death medicine.anatomical_structure Linear Models Solvents Bone marrow Autopsy Gas chromatography–mass spectrometry 0210 nano-technology medicine.drug |
| Zdroj: | Talanta. 191 |
| ISSN: | 1873-3573 |
| Popis: | Forensic toxicologists typically work with body fluids, such as blood and urine, or visceral tissues. The analysis of alternative samples, such as bone marrow, can be requested when the commonly used samples are unavailable due to an extended time lapse between the time of death and collection of the material to be analysed. In this study, a method for the analysis of the lipophilic drug famprofazone (FA) and its metabolites, methamphetamine (MA) and amphetamine (AM), in bone marrow was developed, validated and applied to bone marrow from pigs given controlled doses of famprofazone. This method involves enzymatic bone-cleaning, fragmentation of the bones with the assistance of a micro electric motor, optimization of clean-up and LLE (liquid/liquid extraction) conditions and determination by GC/MS. After evaluation through statistical tests, such as Shapiro Wilk for normality and Cochran for homoscedasticity, a linear model was applied in the range of 100 (LOQ) – 2000 ng g−1. Inter-day precision and bias was always |
| Databáze: | OpenAIRE |
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