Exploration of scalp surface lipids reveals squalene peroxide as a potential actor in dandruff condition
| Autor: | Alain Moga, Nicolas Amalric, Charles El Rawadi, Florence Pouradier, Philippe Vingler, Roland Jourdain, Luc Souverain, Lionel Breton, Philippe Bastien |
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| Rok vydání: | 2016 |
| Předmět: |
Keratinocytes
Male 0301 basic medicine Dandruff scalp medicine.disease_cause Cohort Studies Lipid peroxidation 030207 dermatology & venereal diseases Squalene chemistry.chemical_compound 0302 clinical medicine Malondialdehyde Barrier function integumentary system biology Fatty Acids General Medicine Middle Aged Lipids Healthy Volunteers medicine.anatomical_structure Biochemistry Female Malassezia medicine.symptom Adult medicine.medical_specialty Dermatology Gas Chromatography-Mass Spectrometry 03 medical and health sciences Internal medicine medicine Humans Original Paper Scalp Dandruff biology.organism_classification Sebum Oxidative Stress 030104 developmental biology Endocrinology chemistry Squalene peroxide Lipid Peroxidation Biomarkers Oxidative stress |
| Zdroj: | Archives of Dermatological Research |
| ISSN: | 1432-069X 0340-3696 |
| DOI: | 10.1007/s00403-016-1623-1 |
| Popis: | Dandruff is a common but complex disorder with three major contributing factors: (1) individual predisposition, (2) scalp sebum and (3) Malassezia yeast colonization. To obtain further insights into the role of sebum in dandruff biogenesis, we analyzed scalp lipid species in a cohort of ten dandruff-free (control) and ten dandruff-afflicted volunteers by gas chromatography coupled to mass spectrometry. Lipid peroxidation levels and biochemical markers of oxidative stress were also assessed. Squalene, a major sebum component, was significantly more peroxidized in dandruff-affected scalps, resulting in significantly higher ratios of squalene monohydroperoxide (SQOOH)/squalene. This was observed when comparing dandruff-affected zones of dandruff subjects to both their non-affected zones and control subjects. In addition, other biomarkers such as malondialdehyde indicated that oxidative stress levels were raised on dandruff scalps. Surprisingly, differences regarding either free or bound fatty acids were fairly rare and minor. Certain novel findings, especially squalene peroxidation levels, were then confirmed in a validation cohort of 24 dandruff-affected subjects, by comparing dandruff-affected and non-dandruff zones from the same individuals. As SQOOH can induce both keratinocyte inflammatory responses and hyperproliferation in vitro, we hypothesized that increased SQOOH could be considered as a new etiological dandruff factor via its ability to impair scalp barrier function. Our results also indicated that Malassezia could be a major source of squalene peroxidation on the scalp. Electronic supplementary material The online version of this article (doi:10.1007/s00403-016-1623-1) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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