Design of Dual-Emission Chemosensors for Ratiometric Detection of ATP Derivatives
| Autor: | Itaru Hamachi, Yoshifumi Miyahara, Kazuki Sada, Akio Ojida, Shun Ichi Tamaru, Jirarut Wongkongkatep |
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| Rok vydání: | 2006 |
| Předmět: |
Anions
Fluorophore Stereochemistry Chemistry Pharmaceutical enzymes Molecular Conformation sensors Biochemistry Pyrophosphate Catalysis Fluorescence Coordination complex chemistry.chemical_compound Adenosine Triphosphate fluorescent probes Glycosyl Fluorescent Dyes chemistry.chemical_classification Aqueous solution Organic Chemistry Nucleosides General Chemistry Diphosphates Kinetics Spectrometry Fluorescence Models Chemical chemistry Acridine coordination chemistry Nucleoside |
| Zdroj: | Chemistry – An Asian Journal. 1:555-563 |
| ISSN: | 1861-471X 1861-4728 |
| Popis: | Nucleoside pyrophosphate (nucleoside PP) derivatives are widespread in living cells and play pivotal roles in various biological events. We report novel fluorescence chemosensors for nucleoside PPs that make use of coordination chemistry. The chemosensors, which contain two Zn(II)-dipicolylamine units, bind strongly to nucleoside PPs (K(app)>10(6) M(-1)) in aqueous solution and sense them by a dual-emission change. Detailed fluorescence and UV/Vis spectral studies revealed that the emission changes of the chemosensors upon binding to nucleoside PPs can be ascribed to the loss of coordination between Zn(II) and the acridine fluorophore. This is a unique sensing system based on the anion-induced rearrangement of the coordination. Furthermore, we demonstrated the utility of these chemosensors in real-time monitoring of two important biological processes involving nucleoside PP conversion: the apyrase-catalyzed hydrolysis of nucleoside PPs and the glycosyl transfer catalyzed by beta-1,4-galactosyltransferase. |
| Databáze: | OpenAIRE |
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