Vector separation of particles and cells using an array of slanted open cavities
| Autor: | Konstantinos Konstantopoulo, Jorge A. Bernate, Liesbet Lagae, Chengxun Liu, German Drazer |
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| Rok vydání: | 2014 |
| Předmět: |
Materials science
Flow (psychology) Microfluidics Biomedical Engineering FOS: Physical sciences Bioengineering 02 engineering and technology Cell Separation Condensed Matter - Soft Condensed Matter 01 natural sciences Biochemistry Article Settling Leukocytes Humans Particle Size Range (particle radiation) Plane (geometry) 010401 analytical chemistry Fluid Dynamics (physics.flu-dyn) Physics - Fluid Dynamics General Chemistry Mechanics Microfluidic Analytical Techniques 021001 nanoscience & nanotechnology Silicon Dioxide 0104 chemical sciences Volumetric flow rate Deflection (physics) Polystyrenes Soft Condensed Matter (cond-mat.soft) 0210 nano-technology Parallel array |
| DOI: | 10.48550/arxiv.1404.1277 |
| Popis: | We present a microfluidic platform for the continuous separation of suspended particles based on their size and settling velocity. The separation method takes advantage of the flow field in the vicinity and inside a parallel array of slanted open cavities. These cavities induce flow along them, which deflects the suspended particles to a different degree depending on the extent to which they penetrate into the cavities. The cumulative deflection in the periodic array ultimately leads to vector chromatography, with the different species in the sample moving in different directions. We demonstrate density and size based separation over a range of flow rates by separating polystyrene and silica particles and show that purities nearing 100% can be achieved for multicomponent mixtures. We also demonstrate the potential of the platform to separate biological cells by fractionating different blood components. We discuss the presence of two regimes, which can be distinguished depending on the ratio between the settling velocity and the velocity of the particles across the open cavities. The proposed platform could also integrate additional separative force fields in the direction normal to the plane of the cavities to fractionate specific mixtures based on the distinguishing properties of the component species. |
| Databáze: | OpenAIRE |
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