Transcriptome Analysis of the Duodenum, Pancreas, Liver, and Muscle from Diabetic Goto-Kakizaki Rats Fed a Trypsin Inhibitor Derived from Squid Viscera
| Autor: | Hiroki Saeki, Satoru Chiba, Nobuhiro Zaima, Michihiro Araki, Kana Fukumorita, Zhi-Hong Yang, Kohsuke Adachi, Hideki Kishimura |
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| Rok vydání: | 2012 |
| Předmět: |
Blood Glucose
Male medicine.medical_specialty Duodenum Trypsin inhibitor Biology Transcriptome Internal medicine medicine Animals Humans Hypoglycemic Agents Pancreas Pancreatic hormone Gene Expression Profiling Decapodiformes General Chemistry Cholesterol biosynthetic process Trypsin Small intestine Rats Disease Models Animal Viscera medicine.anatomical_structure Endocrinology Diabetes Mellitus Type 2 Liver Organ Specificity Sterol Regulatory Element Binding Protein 1 Trypsin Inhibitors General Agricultural and Biological Sciences medicine.drug |
| Zdroj: | Journal of Agricultural and Food Chemistry. 60:5540-5546 |
| ISSN: | 1520-5118 0021-8561 |
| Popis: | Trypsin inhibitors (TIs) have various nutritional effects. However, a detailed mechanism for their effects, especially on the gene expression patterns in various tissues, remains unknown. Here, we used transcriptome techniques and gene ontology (GO) analysis to examine the effects of squid TI (sqTI), a biochemically stable peptide, on diabetic Goto-Kakizaki rats after feeding for 10 weeks. We demonstrated that downregulation of SREBP1c in the liver via duodenal/pancreatic hormones suppresses the blood cholesterol level. Consistently, in GO analysis, the term "cholesterol biosynthetic process" was enriched among downregulated genes. No hypoglycemic or insulinotropic effects were observed, in contrast to the results from our previous studies (single stimulation with the same dose of TI), which can be partly ascribed to the inactive responses of the duodenum and pancreas in this condition. |
| Databáze: | OpenAIRE |
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