Assessment of tobacco heating product THP1.0. Part 5: In vitro dosimetric and cytotoxic assessment
| Autor: | Anya Terry, Simone Santopietro, David Thorne, Christopher Proctor, Jason Adamson, Marianna Gaça, Damien Breheny, Tomasz Jaunky |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Nicotine Neutral red Cell Survival Statistical difference Respiratory Mucosa Electronic Nicotine Delivery Systems 010501 environmental sciences Toxicology 01 natural sciences Tobacco smoke Cell Line Heating 03 medical and health sciences chemistry.chemical_compound medicine Humans Cytotoxic T cell Food science 0105 earth and related environmental sciences Weight of evidence Dose-Response Relationship Drug Cytotoxins Chemistry Tobacco Products General Medicine In vitro 030104 developmental biology Toxicant medicine.drug |
| Zdroj: | Regulatory Toxicology and Pharmacology. 93:52-61 |
| ISSN: | 0273-2300 |
| DOI: | 10.1016/j.yrtph.2017.09.016 |
| Popis: | Tobacco heating products (THPs) represent a subset of the next-generation nicotine and tobacco product category, in which tobacco is typically heated at temperatures of 250-350 °C, thereby avoiding many of the harmful combustion-related toxicant emissions of conventional cigarettes. In this study, we have assessed aerosol generation and cytotoxicity from two commercially available THPs, THP1.0 and THS, relative to tobacco smoke from 3R4F reference cigarettes, using an adapted Borgwaldt RM20S Smoking Machine. Quantification of nicotine in the exposed cell-culture media showed greater delivery of nicotine from both THPs than from the cigarette. Using Neutral Red Uptake assay, THPs demonstrated reduced in vitro cytotoxicity in H292 human bronchial epithelial cells as compared with 3R4F cigarette exposure at the air-liquid interface (p 0.0001). Both THPs demonstrated a statistically similar reduction in biological response, with87% viability relative to 3R4F at a common aerosol dilution (1:40, aerosol:air). A similar response was observed when plotted against nicotine; a statistical difference between 3R4F and THPs (p 0.0001) and no difference between the THPs (p = 0.0186). This pre-clinical in vitro biological testing forms part of a larger package of data to help assess the safety and risk reduction potential of next-generation tobacco products relative to cigarettes, using a weight of evidence approach. |
| Databáze: | OpenAIRE |
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