Expression of Musashi-1 Increases in Bone Healing
| Autor: | Pablo Galindo-Moreno, Pedro Hernández-Cortés, Nati Martin-Morales, Dario Abril-Garcia, Vicente Crespo-Lora, Clara Candido-Corral, Francisco O'Valle, Miguel Padial-Molina |
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| Přispěvatelé: | [Padial-Molina,M, Martin-Morales,N, Abril-Garcia,D, Galindo-Moreno,P] Department of Oral Surgery and Implant Dentistry, School of Dentistry, and Centre for Biomedical Research (CIBM), University of Granada, Granada, Spain. [Crespo-Lora,V, Candido-Corral,C, Martin-Morales,N] Department of Pathology, University of Granada, Granada, Spain. [Hernandez-Cortes,P] Department of Orthopedic Surgery, San Cecilio University Hospital, Granada, Spain. [O'Valle,F] Department of Pathology, Institute of Biopathology and Regenerative Medicine (IBIMER, CIBM), and Institute of Biosanitary (ibs-Granada), University of Granada, Granada, Spain., The authors of this manuscript were partially supported by Research Groups #CTS-138 and #CTS-1028 (Junta de Andalucía, Spain). |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Male Core Binding Factor Alpha 1 Subunit Anatomy::Cells::Connective Tissue Cells::Chondrocytes [Medical Subject Headings] lcsh:Chemistry 0302 clinical medicine Osteogenesis Runx2 Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Nerve Tissue Proteins [Medical Subject Headings] Organisms::Eukaryota::Animals [Medical Subject Headings] Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Rats::Rats Wistar [Medical Subject Headings] Bony Callus Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Glycoproteins::Membrane Glycoproteins::Cell Adhesion Molecules [Medical Subject Headings] lcsh:QH301-705.5 Spectroscopy Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Carrier Proteins::RNA-Binding Proteins [Medical Subject Headings] Fracture Healing periostin Bone healing Anatomy::Tissues::Connective Tissue::Bone and Bones::Bony Callus [Medical Subject Headings] RNA-Binding Proteins General Medicine Phenomena and Processes::Biological Phenomena::Biological Processes::Regeneration::Wound Healing::Fracture Healing [Medical Subject Headings] Curación de fractura Anatomy::Cells::Connective Tissue Cells::Osteoblasts::Osteocytes [Medical Subject Headings] Computer Science Applications RUNX2 Immunohistochemistry bone healing Anatomy::Cells::Connective Tissue Cells::Osteoblasts [Medical Subject Headings] Células madre mesenquimatosas Nerve Tissue Proteins Periostin Biology Osteocytes Article Catalysis Proteínas de unión al ARN Inorganic Chemistry 03 medical and health sciences Chondrocytes Animals Physical and Theoretical Chemistry Progenitor cell Rats Wistar Bone regeneration Molecular Biology Transcription factor Subunidad alfa 2 del factor de unión al sitio principal mesenchymal stem cells Osteoblasts Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Transcription Factors::Core Binding Factors::Core Binding Factor alpha Subunits::Core Binding Factor Alpha 1 Subunit [Medical Subject Headings] Organic Chemistry Mesenchymal stem cell 030206 dentistry Rats 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 Musashi-1 Cancer research Phenomena and Processes::Musculoskeletal and Neural Physiological Phenomena::Musculoskeletal Physiological Phenomena::Musculoskeletal Physiological Processes::Musculoskeletal Development::Bone Development::Osteogenesis [Medical Subject Headings] Mesenchymal stem cells Proteína Cell Adhesion Molecules |
| Zdroj: | Digibug. Repositorio Institucional de la Universidad de Granada instname International Journal of Molecular Sciences, Vol 22, Iss 3395, p 3395 (2021) International Journal of Molecular Sciences Volume 22 Issue 7 Digibug: Repositorio Institucional de la Universidad de Granada Universidad de Granada (UGR) |
| Popis: | The authors of this manuscript were partially supported by Research Groups #CTS-138 and #CTS-1028 (Junta de Andalucía, Spain). This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors. All experiments were performed after the approval of the Committee on Animal Research of the University of Granada (CEEA 2014/357) and under the European Union and Spanish regulations for ethics in animal research (EU Directive 63/2010 and Spanish RD 53/2013) and reported following the ARRIVE guidelines. The data that support the findings of this study are available from the corresponding author upon reasonable request. The authors would especially like to acknowledge the contribution to the histochemical and immunohistochemical studies of Serafin Vélez García and Carmen Ruíz Guzmán, technicians in the Department of Surgery, and María Dolores Rodriguez, in the Department of Pathology and IBIMER of the University of Granada and Justin G. Davis for assistance with the writing style. Musashi-1 (MSI1) is an RNA-binding protein that regulates progenitor cells in adult and developing organisms to maintain self-renewal capacities. The role of musashi-1 in the bone healing environment and its relation with other osteogenic factors is unknown. In the current study, we analyze the expression of MSI1 in an experimental model of rat femoral bone fractures. We also analyze the relation between MSI1 expression and the expression of two osteogenic markers: periostin (POSTN) and runt-related transcription factor 2 (RUNX2). We use histological, immunohistochemical, and qPCR techniques to evaluate bone healing and the expression of MSI1, POSTN, and RUNX2 over time (4, 7, and 14 days). We compare our findings with non-fractured controls. We find that in bone calluses, the number of cells expressing MSI1 and RUNX2 increase over time and the intensity of POSTN expression decreases over time. Within bone calluses, we find the presence of MSI1 expression in mesenchymal stromal cells, osteoblasts, and osteocytes but not in hypertrophic chondrocytes. After 14 days, the expression of MSI1, POSTN, and RUNX2 was significantly correlated. Thus, we conclude that musashi-1 potentially serves in the osteogenic differentiation of mesenchymal stromal cells and bone healing. Therefore, further studies are needed to determine the possibility of musashi-1 ' s role as a clinical biomarker of bone healing and therapeutic agent for bone regeneration. Junta de Andalucia European Commission CTS-138 CTS-1028 |
| Databáze: | OpenAIRE |
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