Genetic diversity of GBV-C/HGV strains among HIV infected-IVDU and blood donors from Buenos Aires, Argentina
| Autor: | Ramiro E. Verdun, Jorge Quarleri, D. Ferrario, Jose Raul Oubiña, Daniel O. Sánchez, J Fernández, Mariana Feld, O Libonatti, Veronica Lidia Mathet, M. P. della Latta, Guadalupe Carballal |
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| Rok vydání: | 1999 |
| Předmět: |
Untranslated region
Adult Male Cancer Research Argentina Blood Donors HIV Infections Biology Viral Envelope Proteins Virology Genetic variation Humans Genetic Testing Substance Abuse Intravenous Antigens Viral Membrane Glycoproteins Molecular epidemiology Phylogenetic tree Flaviviridae RNA Genetic Variation Amplicon Infectious Diseases GenBank Female Restriction fragment length polymorphism |
| Zdroj: | Virus research. 65(2) |
| ISSN: | 0168-1702 |
| Popis: | GBV-C/HGV RNA was investigated in serum samples from 70 HIV(+) intravenous drug users (IVDU), as well as from 200 blood donors from Buenos Aires, Argentina. Viral RNA was demonstrated in 21 IVDU by reverse transcription-nested PCR of the 5' UTR. c-DNA amplified products were analyzed and their sequences compared with those downloaded from GenBank. A phylogenetic tree based on 171 sequences demonstrated the presence of three major genogroups, including two subgroups, within local samples, i.e. group 1 (n=1), 2a (n=11), 2b (n=4) and 3 (n=5). These results agreed entirely with those obtained by a novel RFLP (J. Clin. Microbiol. 37, 1340-1347, 1999) of the same 5' UTR amplicons. As expected, GBV-C/HGV RNA prevalence was significantly higher among IVDU than among blood donors (P |
| Databáze: | OpenAIRE |
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