Inhalation of lung spheroid cell secretome and exosomes promotes lung repair in pulmonary fibrosis

Autor: Dipti Paudel, Phuong-Uyen Dinh, Kenneth B. Adler, Tyler A. Allen, Kristen D. Popowski, M. Cyndell Gracieux, Lauren V. Schnabel, Ke Huang, Leonard J. Lobo, Raina T. Barrio, Kevin Blackburn, Erin Harrell, Thomas G. Caranasos, Hayden N. Brochu, Shiqi Hu, Michael B. Goshe, Xinxia Peng, Arianna George, M. Taylor Hensley, Ke Cheng, Adam C. Vandergriff, Jhon Cores
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Proteomics
Pathology
General Physics and Astronomy
Apoptosis
Stem cells
Exosomes
Idiopathic pulmonary fibrosis
chemistry.chemical_compound
Mice
0302 clinical medicine
Fibrosis
Pulmonary fibrosis
Medicine
lcsh:Science
Myofibroblasts
Lung
Multidisciplinary
Interstitial lung disease
Lung Injury
respiratory system
Silicon Dioxide
3. Good health
medicine.anatomical_structure
030220 oncology & carcinogenesis
embryonic structures
medicine.medical_specialty
Science
Lung injury
Bleomycin
Exosome
General Biochemistry
Genetics and Molecular Biology
Article
03 medical and health sciences
Spheroids
Cellular
Administration
Inhalation
Animals
Humans
Cell Proliferation
Respiratory tract diseases
business.industry
Mesenchymal Stem Cells
General Chemistry
Translational research
medicine.disease
Idiopathic Pulmonary Fibrosis
respiratory tract diseases
Disease Models
Animal
030104 developmental biology
chemistry
Alveolar Epithelial Cells
lcsh:Q
sense organs
business
Zdroj: Nature Communications, Vol 11, Iss 1, Pp 1-14 (2020)
Nature Communications
ISSN: 2041-1723
Popis: Idiopathic pulmonary fibrosis (IPF) is a fatal and incurable form of interstitial lung disease in which persistent injury results in scar tissue formation. As fibrosis thickens, the lung tissue loses the ability to facilitate gas exchange and provide cells with needed oxygen. Currently, IPF has few treatment options and no effective therapies, aside from lung transplant. Here we present a series of studies utilizing lung spheroid cell-secretome (LSC-Sec) and exosomes (LSC-Exo) by inhalation to treat different models of lung injury and fibrosis. Analysis reveals that LSC-Sec and LSC-Exo treatments could attenuate and resolve bleomycin- and silica-induced fibrosis by reestablishing normal alveolar structure and decreasing both collagen accumulation and myofibroblast proliferation. Additionally, LSC-Sec and LSC-Exo exhibit superior therapeutic benefits than their counterparts derived from mesenchymal stem cells in some measures. We showed that an inhalation treatment of secretome and exosome exhibited therapeutic potential for lung regeneration in two experimental models of pulmonary fibrosis.
Idiopathic pulmonary fibrosis (IPF) is a fatal lung disease and adult lung spheroid cells have been shown to promote regeneration in animal models of IPF. Here the authors show that the secretome and exosomes of lung spheroid cells is effective as inhalation treatment in rodent models of lung injury and fibrosis and superior to the counterparts derived from mesenchymal stem cells.
Databáze: OpenAIRE
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