Pyrosequencing of the CYP2C9 -1766T>C polymorphism as a means of detecting the CYP2C9*8 allele

Autor:	Shitalben R. Patel, Larisa H. Cavallari, Shan S. Wong, Taimour Y. Langaee
Rok vydání:	2014
Předmět:	Pharmacology Genetics Genotype Locus (genetics) Sequence Analysis DNA Biology Polymorphism Single Nucleotide Molecular biology DNA sequencing Homology (biology) Humans Molecular Medicine Pyrosequencing Allele CYP2C9 Gene Genotyping Alleles Cytochrome P-450 CYP2C9
Zdroj:	Pharmacogenomics. 15:1717-1722
ISSN:	1744-8042 1462-2416
Popis:	The CYP2C9 c.449G>A (p.R150H, rs7900194) polymorphism, which confers the CYP2C9*8 allele, is common in persons of African descent and results in reduced clearance of the narrow therapeutic index drugs, warfarin and phenytoin. Because of significant homology in DNA sequence at the 449G>A locus among CYP2C genes, the 449G>A variant cannot be reliably detected via PCR-based genotyping assays that require a short PCR product, such as pyrosequencing. Herein, we propose genotyping for the CYP2C9 c.-1766T>C polymorphism via pyrosequencing as an alternative and accurate means of identifying the CYP2C9*8 allele.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c9c34022ae2f690f5dc5d9f0abbf228 https://doi.org/10.2217/pgs.14.130 Zobrazit plný text záznamu