Ethanol-Responsive Brain Region Expression Networks: Implications for Behavioral Responses to Acute Ethanol in DBA/2J versus C57BL/6J Mice
Autor: | Ajay Ravindranathan, Tim York, Mary P. Cage, Michael F. Miles, Robnet T. Kerns, Sajida Hassan, Robert W. Williams, James M. Sikela |
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Rok vydání: | 2005 |
Předmět: |
Male
Blotting Western Quantitative Trait Loci Gene Expression Prefrontal Cortex Neuropeptide Biology Nucleus accumbens Drug Administration Schedule Nucleus Accumbens Mice Species Specificity Neurobiology of Disease Gene expression Neuroplasticity medicine Animals Prefrontal cortex Behavior Animal Dose-Response Relationship Drug Ethanol Reverse Transcriptase Polymerase Chain Reaction Microarray analysis techniques General Neuroscience Ventral Tegmental Area Central Nervous System Depressants Computational Biology Microarray Analysis Mice Inbred C57BL Ventral tegmental area Gene expression profiling medicine.anatomical_structure Gene Expression Regulation Mice Inbred DBA Multivariate Analysis Neuroscience |
Zdroj: | The Journal of Neuroscience. 25:2255-2266 |
ISSN: | 1529-2401 0270-6474 |
Popis: | Activation of the mesolimbic dopamine reward pathway by acute ethanol produces reinforcement and changes in gene expression that appear to be crucial to the molecular basis for adaptive behaviors and addiction. The inbred mouse strains DBA/2J and C57BL/6J exhibit contrasting acute behavioral responses to ethanol. We used oligonucleotide microarrays and bioinformatics methods to characterize patterns of gene expression in three brain regions of the mesolimbic reward pathway of these strains. Expression profiling included examination of both differences in gene expression 4 h after saline injection or acute ethanol (2 g/kg). Using a rigorous stepwise method for microarray analysis, we identified 788 genes differentially expressed in control DBA/2J versus C57BL/6J mice and 307 ethanol-regulated genes in the nucleus accumbens, prefrontal cortex, and ventral tegmental area. There were strikingly divergent patterns of ethanol-responsive gene expression in the two strains. Ethanol-responsive genes also showed clustering at discrete chromosomal regions, suggesting local chromatin effects in regulation. Ethanol-regulated genes were generally related to neuroplasticity, but regulation of discrete functional groups and pathways was brain region specific: glucocorticoid signaling, neurogenesis, and myelination in the prefrontal cortex; neuropeptide signaling and developmental genes, including factorBdnf, in the nucleus accumbens; and retinoic acid signaling in the ventral tegmental area. Bioinformatics analysis identified several potential candidate genes for quantitative trait loci linked to ethanol behaviors, further supporting a role for expression profiling in identifying genes for complex traits. Brain region-specific changes in signaling and neuronal plasticity may be critical components in development of lasting ethanol behavioral phenotypes such as dependence, sensitization, and craving. |
Databáze: | OpenAIRE |
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