Expression of deafness protein Tmie in postnatal developmental stages of C57BL/6J mice

Autor:	Sankarapandian Karuppasamy, Jun-Gyo Suh, YoonYi Nam, Harry Jung
Rok vydání:	2012
Předmět:	Kidney Pathology medicine.medical_specialty Letter Lung hearing loss disorder Transmembrane inner ear protein Spleen Biology C57bl 6j Blot Andrology Animal model medicine.anatomical_structure C57BL/6J otorhinolaryngologic diseases medicine sense organs Gene Cochlea
Zdroj:	Laboratory Animal Research
ISSN:	2233-7660 1738-6055
DOI:	10.5625/lar.2012.28.2.147
Popis:	Loss-of function mutations in the transmembrane inner ear expressed (Tmie/TMIE) gene have been shown to cause deafness in mice and humans (DFNB6). Previous studies report that the circling mouse can be an animal model for DFNB6. However, the expression pattern of Tmie protein in postnatal developmental stages has not been clearly revealed. In this study we tried to investigate the expression of Tmie protein in the liver, spleen, kidney, and lung, as well as in the cochlea. We examined various tissue samples from five different age groups of C57BL/6J animals. Using western blotting analysis, the expression of Tmie protein in these organs has been identified. The results show that Tmie protein expression in the cochlea has been increased in postnatal developmental stages, indicating that Tmie plays an important role in not only the development and also in the function of the cochlea. The expression pattern of Tmie in adult mouse organs such as the liver, spleen, kidney, and spleen significantly vary in adult rats. The order of Tmie expression level in mice (63 days after birth) was spleen, liver, lung, cochlea, and kidney, whereas in the adult rat it was liver, cochlea, lung, spleen, and kidney.
Databáze:	OpenAIRE
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