Sequential interactions with Sec23 control the direction of vesicle traffic
| Autor: | Deborah C. Nycz, Susan Ferro-Novick, Christopher L. Lord, Jesse C. Hay, Majid Ghassemian, Pradipta Ghosh, Shekar Menon, Deepali Bhandari |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Vesicle fusion Vesicular Transport Proteins Golgi Apparatus Saccharomyces cerevisiae Biology Endoplasmic Reticulum Article Vesicle tethering 03 medical and health sciences symbols.namesake 0302 clinical medicine Animals COPII 030304 developmental biology 0303 health sciences Multidisciplinary Casein Kinase I Endoplasmic reticulum Vesicle Lipid bilayer fusion COPI Golgi apparatus Rats Cell biology symbols COP-Coated Vesicles SNARE Proteins 030217 neurology & neurosurgery |
| Zdroj: | Nature |
| ISSN: | 1476-4687 0028-0836 |
| Popis: | How the directionality of vesicle traffic is achieved remains an important unanswered question in cell biology. The Sec23p/Sec24p coat complex sorts the fusion machinery (SNAREs) into vesicles as they bud from the endoplasmic reticulum (ER). Vesicle tethering to the Golgi begins when the tethering factor TRAPPI binds to Sec23p. Where the coat is released and how this event relates to membrane fusion is unknown. Here we use a yeast transport assay to demonstrate that an ER-derived vesicle retains its coat until it reaches the Golgi. A Golgi-associated kinase, Hrr25p (CK1δ orthologue), then phosphorylates the Sec23p/Sec24p complex. Coat phosphorylation and dephosphorylation are needed for vesicle fusion and budding, respectively. Additionally, we show that Sec23p interacts in a sequential manner with different binding partners, including TRAPPI and Hrr25p, to ensure the directionality of ER-Golgi traffic and prevent the back-fusion of a COPII vesicle with the ER. These events are conserved in mammalian cells. |
| Databáze: | OpenAIRE |
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