Using the chicken genome sequence in the development and mapping of genetic markers in the turkey (Meleagris gallopavo)
| Autor: | Todd P. Knutson, L. D. Chaves, Stacy B. Krueth, Kent M. Reed |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Genetic Markers
Turkeys animal structures Genotype Genetic Linkage Biology Genome law.invention law Genetics Animals Polymerase chain reaction Alleles Whole genome sequencing Polymorphism Genetic Chromosome Mapping General Medicine Genomics biology.organism_classification genomic DNA Genetic marker Microsatellite Animal Science and Zoology Primer (molecular biology) Meleagris gallopavo Chickens Sequence Alignment Microsatellite Repeats |
| Zdroj: | Animal genetics. 37(2) |
| ISSN: | 0268-9146 |
| Popis: | The efficacy of employing the chicken genome sequence in developing genetic markers and in mapping the turkey genome was studied. Eighty previously uncharacterized microsatellite markers were identified for the turkey using BLAST alignment to the chicken genome. The chicken sequence was then used to develop primers for polymerase chain reaction where the turkey sequence was either unavailable or insufficient. A total of 78 primer sets were tested for amplification and polymorphism in the turkey, and informative markers were genetically mapped. Sixty-five (83%) amplified turkey genomic DNA, and 33 (42%) were polymorphic in the University of Minnesota/Nicholas Turkey Breeding Farms mapping families. All but one marker genetically mapped to the position predicted from the chicken genome sequence. These results demonstrate the usefulness of the chicken sequence for the development of genomic resources in other avian species. |
| Databáze: | OpenAIRE |
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