Annexin A2 functions downstream of c‑Jun N‑terminal kinase to promote skin fibroblast cell migration
| Autor: | Xinmei Wu, Qing Wang, Youpei Wang, Meiqin Zheng, Lingxia Pang |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Proteomics Cancer Research Proteome Cell Citric Acid Cycle Biology Biochemistry Diabetes Mellitus Experimental 03 medical and health sciences 0302 clinical medicine Annexin Cell Movement Genetics medicine Animals Humans Molecular Biology Annexin A2 Cells Cultured Skin Wound Healing Kinase c-jun JNK Mitogen-Activated Protein Kinases Cell migration Cell cycle Fibroblasts Cell biology Rats Disease Models Animal 030104 developmental biology medicine.anatomical_structure Diabetes Mellitus Type 1 Oncology 030220 oncology & carcinogenesis Molecular Medicine Signal transduction Glycolysis Signal Transduction |
| Zdroj: | Molecular medicine reports. 15(6) |
| ISSN: | 1791-3004 |
| Popis: | Delayed healing of skin wounds is one of the outcomes of diabetes mellitus (DM), a condition that affects a significant number of patients worldwide. However, the underlying mechanisms remain unknown. In order to examine proteome alterations in DM, a rat model of type 1 diabetes was developed using streptozotocin injections. The proteomic responses of normal and DM rat skin were analyzed by two‑dimensional electrophoresis, and differentially expressed proteins were identified using a liquid chromatography/mass spectrometry system. DM induced 36 and repressed 41 differentially expressed proteins, respectively. Altered proteins were involved in a number of biological processes, including RNA and protein metabolism, the tricarboxylic acid cycle, glycolysis, cytoskeleton regulation, hydrogen detoxification and calcium‑mediated signal transduction. In addition, overexpression of annexin A2, one of the signaling proteins altered by DM, accelerated the rate of human skin fibroblast cell migration. Application of SP600125, an inhibitor of a key regulator of cell migration c‑Jun N‑terminal kinase (JNK), inhibited the migration of normal cells. By contrast, SP600125 treatment did not inhibit the migration of annexin A2‑overexpressed cells, indicating that annexin A2 may function downstream of JNK. In conclusion, the results of the present study reveal the potential proteomic responses to DM in skin tissues, and demonstrate a positive functional role of annexin A2 in fibroblast cell migration. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|