The detection of Mycoplasma (formerly Eperythrozoon) wenyonii by 16S rDNA PCR and denaturing gradient gel electrophoresis
| Autor: | A. M. Barlow, Suzanna Bell, J. R. Lawes, Robin A.J. Nicholas, Laura McAuliffe, Roger D. Ayling |
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| Rok vydání: | 2006 |
| Předmět: |
DNA
Bacterial Time Factors Molecular Sequence Data Mycoplasmataceae medicine.disease_cause DNA Ribosomal Polymerase Chain Reaction Sensitivity and Specificity Microbiology law.invention Mycoplasma Species Specificity law RNA Ribosomal 16S medicine Animals Mycoplasma Infections Ribosomal DNA Phylogeny Polymerase chain reaction Electrophoresis Agar Gel Base Sequence General Veterinary biology fungi food and beverages General Medicine biology.organism_classification Anaplasmataceae Mollicutes Rickettsiales Temperature gradient gel electrophoresis |
| Zdroj: | Veterinary Microbiology. 117:292-296 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/j.vetmic.2006.06.011 |
| Popis: | Although the role of Mycoplasma wenyonii in disease is still subject to some debate, infections have been reported to result in parasitaemia, anaemia, scrotal and hind limb oedema, tachycardia, pyrexia, infertility, swollen teats, prefemoral lymphadenopathy and decreased milk production. Previously, diagnosis of M. wenyonii has been based on blood smears but is not specific for M. wenyonii and can be difficult to interpret. We have previously described the use of PCR and denaturing gradient gel electrophoresis (DGGE) for the detection and differentiation of Mycoplasma species. DGGE enables the rapid and specific identification of Mycoplasma species and is ideally suited to detecting both mixed infections and new and unusual species. In this study, we have used DGGE with universal primers to detect M. wenyonii DNA from blood samples. DGGE can be used on blood samples as a rapid and specific test for M. wenyonii and can also be used as a screening test for other blood borne pathogens. |
| Databáze: | OpenAIRE |
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