Stem cell separation in paediatric patients
| Autor: | Bernd Neidhardt, Jürgen Zingsem, Jörn D. Beck, Reinhold Eckstein, Robert Zimmermann, Johann Greil, Volker Weisbach |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
business.industry Human albumin solution Immunology Urology CD34 Hematopoietic Stem Cell Transplantation Blood volume Antigens CD34 Hematology Blood flow Peripheral blood mononuclear cell Surgery Blood Cell Count Cobe spectra Medicine Humans Leukapheresis Stem cell business Child Paediatric patients |
| Zdroj: | Transfusion science. 17(4) |
| ISSN: | 0955-3886 |
| Popis: | We performed 28 separations in 11 children weighing from 14 to 72kg (median: 27kg) using either the Cobe Spectra or the Fresenius AS 104 cell separator. For children under 20kg, human albumin solution was used for the last 200 mL of the priming procedure instead of NaCl. Blood flow was reduced to 30–50 mL min −1 depending on the children's size and weight to prevent citrate reactions. Within 149–337 min we processed three times the patients' total blood volume and collected 309.7 × 10 6 (102.9–1140.0) mononuclear cells (MNC) and 2.01 × 10 6 (1.90–6.50) CD34+ cells per kg body weight. These results were comparable to the results we previously obtained in adults. We conclude from these initial results that peripherae blood stem cells (PBSC) separations, with both devices, can be adapted successfully to the needs of paediatric patients. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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