Human Coronavirus NL63 Molecular Epidemiology and Evolutionary Patterns in Rural Coastal Kenya

Autor: Charles N. Agoti, Grieven P. Otieno, Patrick K. Munywoki, Anne Bett, Matthew Cotten, D. James Nokes, Patience K. Kiyuka, Regina Njeru, Taane G. Clark, Paul Kellam, László van den Hoek, Everlyn Kamau, James R. Otieno
Přispěvatelé: AII - Infectious diseases, Medical Microbiology and Infection Prevention, Virology
Rok vydání: 2018
Předmět:
PNEUMONIA
Male
0301 basic medicine
RJ101
RESPIRATORY SYNCYTIAL VIRUS
coronavirus
medicine.disease_cause
TRACT INFECTIONS
Coronavirus OC43
Human
Genotype
Prevalence
ANTIBODY-MEDIATED ENHANCEMENT
Immunology and Allergy
Prospective Studies
Child
Clade
Respiratory Tract Infections
Phylogeny
Coronavirus
Molecular Epidemiology
BIRTH COHORT
biology
Respiratory tract infections
VIRAL-INFECTIONS
virus diseases
11 Medical And Health Sciences
respiratory system
Biological Evolution
3. Good health
Hospitalization
Infectious Diseases
Child
Preschool
Viral evolution
Viruses
Female
Coronavirus Infections
Life Sciences & Biomedicine
RECEPTOR-BINDING
Pneumonia (non-human)
Adult
Human coronavirus NL63
YOUNG-CHILDREN
Adolescent
Immunology
KILIFI DISTRICT
Microbiology
Major Articles and Brief Reports
Young Adult
03 medical and health sciences
repeat infection
stomatognathic system
medicine
Humans
virus evolution
Science & Technology
Molecular epidemiology
HUMAN METAPNEUMOVIRUS
Infant
Newborn
Infant
06 Biological Sciences
biology.organism_classification
medicine.disease
Kenya
Virology
Coronavirus NL63
Human
030104 developmental biology
RC
Zdroj: Journal of infectious diseases, 217(11), 1728-1739. Oxford University Press
The Journal of Infectious Diseases
Journal of Infectious Diseases, 217(11), 1728-1739. Oxford University Press
ISSN: 1537-6613
0022-1899
DOI: 10.1093/infdis/jiy098
Popis: Background Human coronavirus NL63 (HCoV-NL63) is a globally endemic pathogen causing mild and severe respiratory tract infections with reinfections occurring repeatedly throughout a lifetime. Methods Nasal samples were collected in coastal Kenya through community-based and hospital-based surveillance. HCoV-NL63 was detected with multiplex real-time reverse transcription PCR, and positive samples were targeted for nucleotide sequencing of the spike (S) protein. Additionally, paired samples from 25 individuals with evidence of repeat HCoV-NL63 infection were selected for whole-genome virus sequencing. Results HCoV-NL63 was detected in 1.3% (75/5573) of child pneumonia admissions. Two HCoV-NL63 genotypes circulated in Kilifi between 2008 and 2014. Full genome sequences formed a monophyletic clade closely related to contemporary HCoV-NL63 from other global locations. An unexpected pattern of repeat infections was observed with some individuals showing higher viral titers during their second infection. Similar patterns for 2 other endemic coronaviruses, HCoV-229E and HCoV-OC43, were observed. Repeat infections by HCoV-NL63 were not accompanied by detectable genotype switching. Conclusions In this coastal Kenya setting, HCoV-NL63 exhibited low prevalence in hospital pediatric pneumonia admissions. Clade persistence with low genetic diversity suggest limited immune selection, and absence of detectable clade switching in reinfections indicates initial exposure was insufficient to elicit a protective immune response.
Infections with human coronavirus NL63 are common and reinfections occur repeatedly throughout life. A subset of repeat infections show enhanced virus replication with no evidence of genotype switching, indicating that initial exposure is insufficient to elicit a protective immune response.
Databáze: OpenAIRE
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