Distinguishing normal and aggregated alpha-synuclein interaction on gold nanorod incorporated zinc oxide nanocomposite by electrochemical technique
| Autor: | N. A. Parmin, M. K. Md Arshad, Uda Hashim, Subash C. B. Gopinath, Hussaini Adam |
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| Rok vydání: | 2020 |
| Předmět: |
Microscope
Scanning electron microscope Surface Properties chemistry.chemical_element 02 engineering and technology Zinc Protein aggregation Electrochemistry Microscopy Atomic Force Biochemistry Sensitivity and Specificity law.invention Nanocomposites 03 medical and health sciences chemistry.chemical_compound Protein Aggregates Structural Biology law Humans Particle Size Molecular Biology Electrodes 030304 developmental biology Detection limit Alpha-synuclein 0303 health sciences Spin coating Nanotubes Parkinson Disease General Medicine Electrochemical Techniques 021001 nanoscience & nanotechnology chemistry Biophysics Microscopy Electron Scanning alpha-Synuclein Gold Zinc Oxide 0210 nano-technology Aluminum |
| Zdroj: | International journal of biological macromolecules. 171 |
| ISSN: | 1879-0003 |
| Popis: | Misfolding and accumulation of the protein alpha synuclein in the brain cells characterize Parkinson's disease (PD). Electrochemical based aluminum interdigitated electrodes (ALIDEs) was fabricated by using conventional photolithography method and modified the surfaces with zinc oxide and gold nanorod by using spin coating method for the analysis of PD protein biomarker. The device surface modified with gold nanorod of 25 nm diameter was used. The bare devices and the surface modified devices were characterized by Scanning Electron Microscope, 3D-Profilometer, Atomic Force Microscope and high-power microscope. The above measurement was also performed to measure the interaction of antibody with aggregated alpha-synuclein for normal, aggregated and aggregated alpha synuclein in human serum and distinguished against 3 control proteins (PARK1, DJ-1 and Factor IX). The detection limit for normal alpha synuclein was 1 f. with the sensitivity of 1 f. on a linear regression (R2 = 0.9759). The detection limit for aggregated alpha synuclein was 10 aM with the sensitivity of 1 aM on a linear regression (R2 = 0.9797). Also, the detection limit of aggregated alpha synuclein in serum was 10 aM with the sensitivity of 1 aM on a linear regression (R2 = 0.9739). These results however indicate that, serum has only minimal amount of alpha synuclein. |
| Databáze: | OpenAIRE |
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