Tumor cell uptake of 99mTc-labeled 1-thio-β-D-glucose and 5-thio-D-glucose in comparison with 2-deoxy-2-[18F]fluoro-D-glucose in vitro: kinetics, dependencies, blockage and cell compartment of accumulation
| Autor: | G Ulrich, K. H. Smalla, F. L. Muehlberg, K. Mohnike, R. Seidensticker, Jürgen Goldschmidt, Ingo G. Steffen, J. Ricke, O. S. Grosser, Annette Pethe, Holger Amthauer, M. Stiebler, Max Seidensticker |
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| Rok vydání: | 2013 |
| Předmět: |
Cancer Research
Time Factors Phloretin Cytochalasin B medicine.medical_treatment Cell Carbohydrate metabolism chemistry.chemical_compound D-Glucose Fluorodeoxyglucose F18 Cell Line Tumor medicine Humans Insulin Radiology Nuclear Medicine and imaging Technetium Glucose analog Carbohydrate Cell Compartmentation medicine.anatomical_structure Glucose Oncology chemistry Biochemistry Cell culture Radiopharmaceuticals |
| Zdroj: | Molecular imaging and biology. 16(2) |
| ISSN: | 1860-2002 |
| Popis: | This study was conducted to investigate the capacity of (99m)Tc-labeled 1-thio-β-D-glucose ((99m)Tc-1-TG) and 5-thio-D-glucose ((99m)Tc-5-TG) to act as a marker for glucose metabolism in tumor cells in vitro.We investigated the cellular uptake of (99m)Tc-1-TG, (99m)Tc-5-TG, and 2-deoxy-2-[(18)F]fluoro-D-glucose((18)F-FDG) in a human colorectal carcinoma and human lung adenocarcinoma cell line (HCT-116, A549) at different time points and varying glucose/insulin concentrations and under transporter blockage by cytochalasin-B and phloretin. Cell compartment analysis was performed.A significant uptake and time dependency thereof, a significant uptake dependency on glucose and insulin and a significant uptake inhibition by cytochalasin-B for (99m)Tc-1-TG and (99m)Tc-5-TG, was shown. Albeit substantial, the uptake was less pronounced in (99m)Tc-1-TG and (99m)Tc-5-TG compared with (18)F-FDG. (99m)Tc-1-TG and (99m)Tc-5-TG showed a higher accumulation in the cell membranes compared with (18)F-FDG.Tc-1-TG and (99m)Tc-5-TG showed an uptake in vitro with glucose analog characteristics but with membranous accumulation. Tumor imaging should be investigated in an animal model. |
| Databáze: | OpenAIRE |
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