A triticale tapetal non-specific lipid transfer protein (nsLTP) is translocated to the pollen cell wall
| Autor: | Christine Gagnon, Stephen J. B. O’Leary, Shaobo Wu, Claudia Sheedy, Keith Hubbard, Steve Gleddie, Denise Chabot, Mohsin A. Zaidi, Tara Vucurevich, Dhuha Hassan, Dave Sprott, Frances Tran, André Laroche, Laurian S. Robert |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Signal peptide tapetum Stamen Flowers Plant Science Biology nsLTP medicine.disease_cause 01 natural sciences 03 medical and health sciences Microspore Cell Wall Gene Expression Regulation Plant Pollen Locule medicine Cysteine Triticeae triticale Phylogeny Plant Proteins Tapetum food and beverages General Medicine Plants Genetically Modified biology.organism_classification Cell biology Protein Transport 030104 developmental biology pollen liquid transfer protein Agronomy and Crop Science Plant lipid transfer proteins 010606 plant biology & botany Brachypodium |
| DOI: | 10.1007/s00299-020-02556-6 |
| Popis: | A Triticeae type III non-specific lipid transfer protein (nsLTP) was shown for the first time to be translocated from the anther tapetum to the pollen cell wall. Two anther-expressed non-specific lipid transfer proteins (nsLTPs) were identified in triticale (× Triticosecale Wittmack). LTPc3a and LTPc3b contain a putative signal peptide sequence and eight cysteine residues in a C-Xn-C-Xn-CC-Xn-CXC-Xn-C-Xn-C pattern. These proteins belong to the type III class of nsLTPs which are expressed exclusively in the inflorescence of angiosperms. The level of LTPc3 transcript in the anther was highest at the tetrad and uninucleate microspore stages, and absent in mature pollen. In situ hybridization showed that LTPc3 was expressed in the tapetal layer of the developing triticale anther. The expression of the LTPc3 protein peaked at the uninucleate microspore stage, but was also found to be associated with the mature pollen. Accordingly, an LTPc3a::GFP translational fusion expressed in transgenic Brachypodium distachyon first showed activity in the tapetum, then in the anther locule, and later on the mature pollen grain. Altogether, these results represent the first detailed characterization of a Triticeae anther-expressed type III nsLTP with possible roles in pollen cell wall formation. |
| Databáze: | OpenAIRE |
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