Upregulation of complement C1q reflects mucosal regeneration in a mouse model of colitis

Autor: Kyoko Katakura, Naohiko Gunji, Kazumasa Kawashima, Michio Onizawa, Tatsuo Fujiwara, Kazumichi Abe, Hiromasa Ohira, Atsushi Takahashi
Rok vydání: 2020
Předmět:
Zdroj: Medical Molecular Morphology. 54:87-94
ISSN: 1860-1499
1860-1480
DOI: 10.1007/s00795-020-00266-2
Popis: Confirming mucosal healing is important in inflammatory bowel disease treatment. Complement C1q-mediated Wnt signaling activation has recently been suggested to mediate tissue repair and mucosal regeneration. We investigated the involvement of complement C1q and Wnt signaling in intestinal mucosal regeneration using a murine colitis model. The colitis model was established by providing C57BL/6J mice with 4% dextran sodium sulfate (DSS) for 1 week (inflammation phase) followed by regular water for 2 weeks (recovery phase). After 3 weeks, we investigated the relationship between C1q in serum and colonic tissue during the inflammation and recovery phases. We assessed Wnt signaling activity by evaluating β-catenin expression in mouse intestinal tissue. Serum C1q levels were elevated during the recovery phase. C1q-specific staining indicated high C1q expression in pathological intestinal tissue during the inflammation and recovery phases. C1q mRNA and protein expression was increased during both phases. Interestingly, C1q-expressing cells were consistent with macrophages (F4/80-positive cells). Moreover, the expression of β-catenin increased in the colonic tissues during the recovery phase of DSS-induced colitis but decreased during the inflammation phase of DSS-induced colitis. C1q expression may mediate Wnt signaling activity and intestinal epithelial regeneration.
Databáze: OpenAIRE
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