Time-dependent folding of immunological epitopes of the human chorionic gonadotropin beta-subunit
| Autor: | Wolfgang E. Merz, Jean-Michel Krause, Peter Berger, Jordi Roig |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Protein Folding
Time Factors medicine.drug_class Protein subunit Biology Monoclonal antibody Biochemistry Epitope Human chorionic gonadotropin Epitopes Endocrinology medicine Humans Immunoprecipitation Chorionic Gonadotropin beta Subunit Human Molecular Biology Cystine knot Choriocarcinoma Antibodies Monoclonal medicine.disease Molecular biology Protein Subunits Epitope mapping Glycoprotein Hormones alpha Subunit Dimerization Protein Processing Post-Translational Intracellular HeLa Cells |
| Zdroj: | Molecular and cellular endocrinology. |
| ISSN: | 0303-7207 |
| Popis: | We have explored the possibility to use 14 different monoclonal antibodies in order to follow the formation of the respective epitopes during the biosynthesis of hCG subunits and their association in JEG-3 choriocarcinoma cells using pulse (30s to 5 min)-chase (0-180 min) experiments. We found central cystine knot epitope structures (epitope beta1) to be formed immediately and simultaneously with epitopes on the protruding hCG-beta loops 1 and 3. We found also differences in the time-dependent folding of beta2 and beta4 epitopes, which are highly overlapping structures on the loops 1+3. These differences were reinforced by decreasing the temperature during the pulse-chase experiments to 25 degrees C. Moreover, we describe for the first time an intracellular intact hCG beta-subunit form that showed the transient expression of the hCG-beta-core fragment epitope beta11 in the course of the maturation of this subunit which casts new light on the presence of hCG-beta-core fragment in Down's syndrome, tumors and pregnancy. |
| Databáze: | OpenAIRE |
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