Kinetic-dependent enzyme-linked immunosorbent assay for detection of antibodies to Legionella pneumophila
| Autor: | J S Sampson, V C Tsang, B J Brake, H W Wilkinson |
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| Rok vydání: | 1983 |
| Předmět: |
Microbiology (medical)
Serotype Legionella Fluorescent Antibody Technique Enzyme-Linked Immunosorbent Assay Immunofluorescence Legionella pneumophila Microbiology Immunoenzyme Techniques Antigen medicine Humans Serotyping chemistry.chemical_classification medicine.diagnostic_test biology bacterial infections and mycoses medicine.disease biology.organism_classification Antibodies Bacterial Kinetics Enzyme chemistry biology.protein Legionnaires' disease Legionnaires' Disease Antibody Research Article |
| Zdroj: | Journal of Clinical Microbiology. 18:1340-1344 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | A semiautomated, kinetic-dependent, enzyme-linked immunosorbent assay (K-ELISA) was adapted to detect serum antibodies to Legionella pneumophila. In a comparative study, 158 human serum samples (79 pairs) were tested by K-ELISA and the standard indirect immunofluorescence assay for determination of antibody levels to L. pneumophila serogroup 1. K-ELISA determinations were made by using a serogroup-specific antigen or a preparation (unfractionated antigen) which contained both common antigen and serogroup-specific reactivity. There was good correlation between the immunofluorescence assay and the K-ELISA by using either antigen, although greater correlation was achieved with the unfractionated antigen (coefficients of correlation, 0.894 with unfractionated antigen and 0.841 with serogroup-specific antigen). These results indicate that the K-ELISA is a reliable alternative to the immunofluorescence assay for serologically diagnosing legionellosis. |
| Databáze: | OpenAIRE |
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