Human CD46 aberrant splicing in transgenic mice
| Autor: | Marirosa Mora, Mara Rossini, Lubbertus C.F. Mulder |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Genetically modified mouse
Transcription Genetic Sequence analysis Transgene RNA Splicing Molecular Sequence Data Mice Transgenic Biology Transfection Polymerase Chain Reaction Membrane Cofactor Protein Mice Antigens CD RNA analysis Genetics Animals Humans RNA Messenger Transgenes Aberrant splicing Cloning Molecular Gene Membrane Glycoproteins CD46 RNA General Medicine 3T3 Cells Sequence Analysis DNA Blotting Northern Molecular biology Liver HeLa Cells |
| Zdroj: | Gene. 186(1) |
| ISSN: | 0378-1119 |
| Popis: | RNA analysis of mice transgenic for human CD46 reveals almost undetectable levels of the expected transcript and the accumulation of a 900 nt shorter species. cDNA cloning and sequence analysis of this variant demonstrate an aberrant splicing of the transgene RNA. This aberrant splicing is present at low levels in cells that normally express the gene, suggesting that the transgene is lacking regulatory elements influencing the abundance of the shorter transcript. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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