Evaluation of a Method for Rapid Detection of Listeria monocytogenes in Dry-Cured Ham Based on Impedanciometry Combined with Chromogenic Agar
| Autor: | Antonio Herrera, Consuelo Pérez, María C. Rota, Mirian Labrador, Susana Bayarri |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
food.ingredient Listeria 030106 microbiology Colony Count Microbial medicine.disease_cause 01 natural sciences Microbiology Rapid detection 03 medical and health sciences food Listeria monocytogenes medicine Agar Food science Dry cured biology Chromogenic Chemistry 010401 analytical chemistry biology.organism_classification 0104 chemical sciences Meat Products Consumer Product Safety Food Microbiology Pure culture Dried meat Food Science |
| Zdroj: | Journal of Food Protection. 81:705-712 |
| ISSN: | 0362-028X |
| DOI: | 10.4315/0362-028x.jfp-17-380 |
| Popis: | The food industry is in need of rapid, reliable methodologies for the detection of Listeria monocytogenes in ready-to-eat products, as an alternative to the International Organization of Standardization (ISO) 11290-1 reference method. The aim of this study was to evaluate impedanciometry combined with chromogenic agar culture for the detection of L. monocytogenes in dry-cured ham. The experimental setup consisted in assaying four strains of L. monocytogenes and two strains of Listeria innocua in pure culture. The method was evaluated according to the ISO 16140:2003 standard through a comparative study with the ISO reference method with 119 samples of dry-cured ham. Significant determination coefficients (R2 of up to 0.99) for all strains assayed in pure culture were obtained. The comparative study results had 100% accuracy, 100% specificity, and 100% sensitivity. Impedanciometry followed by chromogenic agar culture was capable of detecting 1 CFU/25 g of food. L. monocytogenes was not detected in ... |
| Databáze: | OpenAIRE |
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