A four parameter optimization and troubleshooting of a RPLC - charged aerosol detection stability indicating method for determination of S-lysophosphatidylcholines in a phospholipid formulation
| Autor: | Andrei Blasko, James Tam, Imad A. Haidar Ahmad |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Chemistry
Pharmaceutical Phosphorylcholine Clinical Biochemistry Phase (waves) Analytical chemistry Pharmaceutical Science 02 engineering and technology 01 natural sciences Analytical Chemistry Drug Discovery Power function Spectroscopy Evaporator Chromatography High Pressure Liquid Phospholipids Power density Response factor Aerosols Chromatography Reverse-Phase Chemistry 010401 analytical chemistry Detector Temperature Linearity Lysophosphatidylcholines 021001 nanoscience & nanotechnology 0104 chemical sciences Aerosol 0210 nano-technology Hydrophobic and Hydrophilic Interactions |
| Zdroj: | Journal of pharmaceutical and biomedical analysis. 155 |
| ISSN: | 1873-264X |
| Popis: | A four parameter optimization of a stability indicating method for non-chromophoric degradation products of 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), 1-stearoyl-sn-glycero-3-phosphocholine and 2-stearoyl-sn-glycero-3-phosphocholine was achieved using a reverse phase liquid chromatography-charged aerosol detection (RPLC-CAD) technique. Using the hydrophobic subtraction model of selectivity, a core-shell, polar embedded RPLC column was selected followed by gradient-temperature optimization, resulting in ideal relative peak placements for a robust, stability indicating separation. The CAD instrument parameters, power function value (PFV) and evaporator temperature were optimized for lysophosphatidylcholines to give UV absorbance detector-like linearity performance within a defined concentration range. The two lysophosphatidylcholines gave the same response factor in the selected conditions. System specific power function values needed to be set for the two RPLC-CAD instruments used. A custom flow-divert profile, sending only a portion of the column effluent to the detector, was necessary to mitigate detector response drifting effects. The importance of the PFV optimization for each instrument of identical build and how to overcome recovery issues brought on by the matrix effects from the lipid-RP stationary phase interaction is reported. |
| Databáze: | OpenAIRE |
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