Development and Characterization of a Novel Monoclonal Antibody Against Chitinase-like Protein CHID1 Applicable for Immunohistochemistry on Formalin Fixed Paraffin-Embedded Sections
| Autor: | Sergey N Kurochkin, Alexei Gratchev, Roman V Fedotov, A. A. Petrenko, Daria V. Samoilova, Olga V. Kovaleva, Ksenya V Shelekhova |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
medicine.drug_class Immunology Enzyme-Linked Immunosorbent Assay Monoclonal antibody Immunofluorescence Mice 03 medical and health sciences 0302 clinical medicine Chitinase like protein Cell Line Tumor Formaldehyde Neoplasms medicine Animals Humans Immunology and Allergy Hybridomas Paraffin Embedding Predictive marker 030102 biochemistry & molecular biology biology medicine.diagnostic_test Chemistry Chitinases Antibodies Monoclonal Cancer medicine.disease Immunohistochemistry Molecular biology 030220 oncology & carcinogenesis biology.protein Antibody Carrier Proteins Clone (B-cell biology) |
| Zdroj: | Monoclonal Antibodies in Immunodiagnosis and Immunotherapy. 38:12-17 |
| ISSN: | 2167-9436 |
| DOI: | 10.1089/mab.2018.0039 |
| Popis: | CHID1 has been recently described as a predictive marker of different malignant tumors. Thus, monoclonal antibodies (mAbs) for CHID1 detection in different human liquids and in tissues are an important tool for the diagnosis of CHID1-positive cancers. However, only few mAbs have been established to date. In this study we describe the generation of a new hybridoma clone 3D4 producing anti-CHID1 antibodies. 3D4 mAb specifically binds human CHID1 and was successfully used in enzyme-linked immunosorbent assay, immunoblotting, immunofluorescence on paraformaldehyde-fixed cells, and in immunohistochemistry of paraffin-embedded tissue specimens. These results indicate that this new anti-CHID1 mAb 3D4 will be useful in the diagnosis of CHID1-related cancers and is a strong tool for both basic and clinical research on chitinase-like proteins. |
| Databáze: | OpenAIRE |
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