Ultra fast miniaturized real-time PCR: 40 cycles in less than six minutes
| Autor: | Pavel Neuzil, Chunyan Zhang, Sharon Oh, Juergen Pipper, Lang Zhuo |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Silicon
Miniaturization Time Factors Cantilever Temperature Analytical chemistry chemistry.chemical_element Biology Polymerase Chain Reaction Slip (ceramics) Mice Volume (thermodynamics) chemistry visual_art Heat transfer Genetics visual_art.visual_art_medium Methods Online Animals Thin film Square Millimeter Oligonucleotide Array Sequence Analysis |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/gkl416 |
| Popis: | We have designed, fabricated and tested a real-time PCR chip capable of conducting one thermal cycle in 8.5 s. This corresponds to 40 cycles of PCR in 5 min and 40 s. The PCR system was made of silicon micromachined into the shape of a cantilever terminated with a disc. The thin film heater and a temperature sensor were placed on the disc perimeter. Due to the system's thermal constant of 0.27 s, we have achieved a heating rate of 175 degrees C s(-1) and a cooling rate of -125 degrees C s(-1). A PCR sample encapsulated with mineral oil was dispensed onto a glass cover slip placed on the silicon disc. The PCR cycle time was then determined by heat transfer through the glass, which took only 0.5 s. A real-time PCR sample with a volume of 100 nl was tested using a FAM probe. As the single PCR device occupied an area of only a few square millimeters, devices could be combined into a parallel system to increase throughput. |
| Databáze: | OpenAIRE |
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