Fimbrial phase variation in Bordetella pertussis: a novel mechanism for transcriptional regulation
| Autor: | A.R. ter Avest, Frits R. Mooi, H. G. J. van der Heide, A Paul, Rob J. L. Willems |
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| Jazyk: | angličtina |
| Rok vydání: | 1990 |
| Předmět: |
Bordetella pertussis
Genotype Transcription Genetic Molecular Sequence Data Restriction Mapping Locus (genetics) Pertussis toxin General Biochemistry Genetics and Molecular Biology Transcription (biology) Sequence Homology Nucleic Acid Promoter Regions Genetic Molecular Biology Gene Phase variation Genetics General Immunology and Microbiology biology Base Sequence Activator (genetics) General Neuroscience Promoter Gene Expression Regulation Bacterial biology.organism_classification Genes Bacterial Fimbriae Bacterial Oligonucleotide Probes Research Article |
| Zdroj: | ResearcherID |
| Popis: | Fimbriae belong to a class of extracellular filamentous proteins which are involved in the attachment of bacteria to host tissues. Bordetella pertussis, the etiological agent of whooping cough, produces two serologically distinct fimbriae. We show that, like a number of other B. pertussis virulence genes, transcription of the fimbrial subunit genes (fim) is positively controlled by trans-acting polypeptides encoded by the bvg locus. In addition to this coordinate control, transcription of the fim genes is regulated at an individual level by phase variation. This process is characterized by a switching between a high and low level of expression of a particular fim gene. We have identified a conserved DNA region, located close to the start of the fim genes, which is likely to be involved in both positive regulation by the bvg locus, and phase variation. This promoter region contains a stretch of approximately 15 C residues and it appears that phase transitions occur by small insertions or deletions in this C-rich region. We propose that these mutations affect transcription of the fim genes by varying the distance between the binding site for an activator and the -10 box. The fim promoter shows homology with the pertussis toxin promoter, which is also positively regulated by the bvg locus. |
| Databáze: | OpenAIRE |
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