The β-amyloid-related proteins presenilin 1 and BACE1 are axonally transported to nerve terminals in the brain
| Autor: | Vassilis E. Koliatsos, Donald L. Price, Jin G. Sheng |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
medicine.medical_specialty
Blotting Western Perforant Pathway Central nervous system Mice Transgenic Biology Axonal Transport Efferent Pathways Hippocampus Presenilin Lesion Mice Developmental Neuroscience Internal medicine Endopeptidases mental disorders Presenilin-1 medicine Animals Aspartic Acid Endopeptidases Entorhinal Cortex Axon Amyloid beta-Peptides Dentate gyrus Brain Membrane Proteins Axotomy Entorhinal cortex Immunohistochemistry nervous system diseases medicine.anatomical_structure Endocrinology nervous system Neurology Axoplasmic transport Amyloid Precursor Protein Secretases medicine.symptom Neuroscience |
| Zdroj: | Experimental Neurology. 184:1053-1057 |
| ISSN: | 0014-4886 |
| DOI: | 10.1016/j.expneurol.2003.08.018 |
| Popis: | In this study, we show that removal of entorhinal cortex (ERC) afferents to hippocampus reduces levels of presenilin 1 (PS1) in the dentate gyrus of APPswe/PS1ΔE9 transgenic (Tg) mice. PS1 immunoreactivity on the deafferented dentate gyrus decreases by approximately 25% and 50%, 2 and 4 weeks post-lesion compared to the contralateral side; by Western blotting, there is an approximately 40% decrease of the 43 kDa (full length) PS1 and an approximately 80% decrease of the 28 kDa (N-terminal fragment) PS1 on the lesioned dentate gyrus. Levels of β-site APP Cleavage Enzyme 1 (BACE1) immunoreactivity also decrease by approximately 50% and 65% 2 and 4 weeks post-lesion. Together, these data demonstrate that PS1 and BACE1 are transported from the entorhinal cortex to the hippocampus via axons of the perforant pathway. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect