Increase in negative supercoiling of plasmid DNA in Escherichia coli exposed to cold shock
| Autor: | Ryu Ichi Inoue, Kazuhisa Sekimizu, Kazuhiro Kataoka, Yasuyuki Ogata, Tohru Mizushima |
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| Rok vydání: | 1997 |
| Předmět: |
Nalidixic acid
DNA Superhelical HU Protein Biology medicine.disease_cause Microbiology Molecular biology DNA gyrase Cold Temperature DNA-Binding Proteins Nalidixic Acid chemistry.chemical_compound Plasmid Bacterial Proteins chemistry Agarose gel electrophoresis Escherichia coli medicine DNA supercoil Molecular Biology DNA Plasmids medicine.drug |
| Zdroj: | Molecular Microbiology. 23:381-386 |
| ISSN: | 1365-2958 0950-382X |
| DOI: | 10.1046/j.1365-2958.1997.2181582.x |
| Popis: | Negative supercoiling of plasmid DNA in Escherichia coli cells can decrease transiently when exposed to heat shock. The effect of cold shock on DNA supercoiling was examined, and analysis by agarose gel electrophoresis in the presence of chloroquine revealed that negative supercoiling of plasmid DNA in cells increased when cells were exposed to cold shock. This increase was transient and was nil when the cells were pretreated with nalidixic acid, an inhibitor of DNA gyrase. In a mutant deficient in expression of HU protein, the increase in negative supercoiling of DNA by cold shock is less apparent than in wild-type cells. It is proposed that DNA gyrase and HU protein have a role in the DNA supercoiling reaction seen with cold shock. |
| Databáze: | OpenAIRE |
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