Electrochemical Atomic Force Microscopy Imaging of Redox-Immunomarked Proteins on Native Potyviruses: From Subparticle to Single-Protein Resolution
| Autor: | Thierry Michon, Agnès Anne, Daniela Cardinale, Noëlle Carette, Laurent Nault, Arnaud Chovin, Cécilia Taofifenua, Jocelyne Walter, Jane Besong-Ndika, Christophe Demaille |
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| Přispěvatelé: | Laboratoire d'Electrochimie Moléculaire (LEM (UMR_7591)), Université Paris Diderot - Paris 7 (UPD7)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, University of Helsinki |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
In situ
electrochemical atomic force microscopy Surface Properties viruses functional nanosystems General Physics and Astronomy Microscopy Atomic Force Electrochemistry Redox virus imaging Virus potyvirus virus nanocarriers Nanotechnology Molecule General Materials Science [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics biology Chemistry Atomic force microscopy Resolution (electron density) Virion General Engineering Potyvirus biology.organism_classification Crystallography [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology electrochemistry Biophysics Capsid Proteins Gold Microscopy Electrochemical Scanning Oxidation-Reduction |
| Zdroj: | ACS Nano ACS Nano, American Chemical Society, 2015, 9 (5), pp.4911-4924. ⟨10.1021/acsnano.5b00952⟩ |
| ISSN: | 1936-0851 |
| Popis: | International audience; We show herein that electrochemical atomic force microscopy (AFM-SECM), operated in molecule touching (Mt) mode and combined with redox immunomarking, enables the in situ mapping of the distribution of proteins on individual virus particles and makes localization of individual viral proteins possible. Acquisition of a topography image allows isolated virus particles to be identified and structurally characterized, while simultaneous acquisition of a current image allows the sought after protein, marked by redox antibodies, to be selectively located. We concomitantly show that Mt/AFM-SECM, due to its single-particle resolution, can also uniquely reveal the way redox functionalization endowed to viral particles is distributed both statistically among the viruses and spatially over individual virus particles. This possibility makes Mt/AFM-SECM a unique tool for viral nanotechnology. |
| Databáze: | OpenAIRE |
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