Microwave assisted direct saponification for the simultaneous determination of cholesterol and cholesterol oxides in shrimp
| Autor: | Neura Bragagnolo, Lilian Regina Barros Mariutti, Hugo Antonio Lima de Souza |
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| Rok vydání: | 2017 |
| Předmět: |
Chromatography
Gas Endocrinology Diabetes and Metabolism Clinical Biochemistry 01 natural sciences Biochemistry Magnetics chemistry.chemical_compound 0404 agricultural biotechnology Endocrinology Limit of Detection Crustacea Animals Microwaves Molecular Biology Detection limit Ethanol Chromatography 010401 analytical chemistry Extraction (chemistry) Temperature Salting Reproducibility of Results Oxides 04 agricultural and veterinary sciences Cell Biology Repeatability 040401 food science 0104 chemical sciences Shrimp Kinetics Cholesterol chemistry Molecular Medicine Salts Gas chromatography Artifacts Food Analysis Saponification |
| Zdroj: | The Journal of Steroid Biochemistry and Molecular Biology. 169:88-95 |
| ISSN: | 0960-0760 |
| DOI: | 10.1016/j.jsbmb.2016.03.027 |
| Popis: | A novel microwave-assisted direct saponification method for the simultaneous determination of cholesterol and cholesterol oxides in shrimp was developed and validated. Optimal saponification conditions, determined by means of an experimental design, were achieved using 500mg of sample and 20mL of 1mol/L KOH ethanol solution for 16min at 45°C at maximum power at 200W and magnetic stirring at 120rpm. Higher extraction of cholesterol oxides in a reduced saponification time (∼75 times) was achieved in comparison with the direct cold saponification method. The new method showed low detection (≤0.57μg/mL) and quantification (≤1.73μg/mL) limits, good repeatability (≤10.50% intraday and ≤8.56% interday) and low artifact formation (evaluated by using a deuterated cholesterol-D6 standard). Raw, salted and dried-salted shrimps were successfully analyzed by the validated method. The content of cholesterol oxides increased after salting and decreased after drying. |
| Databáze: | OpenAIRE |
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