Screening and characterization of a novel reversible 4-hydroxyisophthalic acid decarboxylase from Cystobasidium slooffiae HTK3
| Autor: | Tomoya Yoshihara, Hotaka Nishida, Kuniki Kino, Riku Aono |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Carboxy-Lyases 030106 microbiology Phthalic Acids Applied Microbiology and Biotechnology Biochemistry Catalysis Analytical Chemistry law.invention Substrate Specificity 03 medical and health sciences law Block (telecommunications) Cystobasidium Molecular Biology Chemistry 4-hydroxyisophthalic acid Basidiomycota Organic Chemistry Temperature General Medicine Combinatorial chemistry Acid production Kinetics 030104 developmental biology Carboxylation Recombinant DNA Biotechnology |
| Zdroj: | Bioscience, biotechnology, and biochemistry. 85(7) |
| ISSN: | 1347-6947 |
| Popis: | Owing to carboxylation activity, reversible decarboxylases can use CO2 as a C1-building block to produce useful carboxylic acids. Although many reversible decarboxylases can synthesize aromatic monocarboxylic acids, only a few reversible decarboxylases have been reported to date that catalyze the synthesis of aromatic dicarboxylic acids. In the present study, a reversible 4-hydroxyisophthalic acid decarboxylase was identified in Cystobasidium slooffiae HTK3. Furthermore, recombinant 4-hydroxyisophthalic acid decarboxylase was prepared, characterized, and used for 4-hydroxyisophthalic acid production from 4-hydroxybenzoic acid. |
| Databáze: | OpenAIRE |
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