Effects of YM 435 and A 77636 on dopamine D-1 receptors in bovine retina in vitro
| Autor: | Olivier Bugnon, Saskia de Raad, Senyo Ofori, Michel Schorderet |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Agonist
medicine.drug_class Adamantane Biology In Vitro Techniques Cyclase Retina A-77636 chemistry.chemical_compound Dopamine Tetrahydroisoquinolines medicine Cyclic AMP Animals Benzopyrans Receptor Pharmacology SCH-23390 Receptors Dopamine D1 Benzazepines Isoquinolines Molecular biology Biochemistry chemistry Dopamine receptor Dopamine Agonists Cattle Signal transduction medicine.drug |
| Zdroj: | General pharmacology. 26(1) |
| ISSN: | 0306-3623 |
| Popis: | 1. 1. Homogenates of bovine retinas were used to study the capacities of two new dopamine (DA) receptor agonists, e.g. YM 435 and A 77636, to interact with D1 receptors. 2. 2. Adenylate cyclase experiments. YM 435 and A 77636 enhanced cAMP accumulation, with EC50 values of 1.97 μM and 22.9 nM, respectively. The receptor-mediated nature of the effects of these two novel D1 agonists was shown by the abilities of both SCH 23390 or (+)-butaclamol to inhibit the agonist-induced increase of cAMP formation. 3. 3. Binding experiments. The same agonists were also tested for competition with 3H-SCH 23390 for binding to D1 receptors. IC50 values for YM 435 and A 77636 were 8.15 μM and 6.15 nM, respectively. 4. 4. The results of the present report demonstrate the suitability of bovine retina in vitro to evaluate the specificity of new DA D1 agonists at the receptor level. |
| Databáze: | OpenAIRE |
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