Arabidopsis thaliana lipid phosphate phosphatase 2 is involved in abscisic acid signalling in leaves
| Autor: | Sophie Paradis, Emmanuelle Jeannette, Yvette Habricot, Estela E. Machado, Emile Miginiac, Bruno Sotta, Susana Saez Aguayo, Christine Zalejski, R. Maldiney, Ana Laura Villasuso |
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| Rok vydání: | 2011 |
| Předmět: |
Diacylglycerol Kinase
Genotype Physiology Phosphatase Arabidopsis Phosphatidate Phosphatase Down-Regulation Gene Expression Phosphatidic Acids Plant Science Biology Genes Plant chemistry.chemical_compound Gene Expression Regulation Plant Stress Physiological Guard cell Genetics Arabidopsis thaliana Abscisic acid Diacylglycerol kinase Phosphotransferases (Phosphate Group Acceptor) Arabidopsis Proteins Kinase organic chemicals fungi Water food and beverages Lipid-phosphate phosphatase Phosphatidic acid biology.organism_classification Adaptation Physiological Droughts chemistry Biochemistry Mutation Plant Stomata Abscisic Acid Signal Transduction |
| Zdroj: | Plant Physiology and Biochemistry. 49:357-362 |
| ISSN: | 0981-9428 |
| DOI: | 10.1016/j.plaphy.2011.01.010 |
| Popis: | Lipid phosphate phosphatases (LPPs, E.C. 3.1.3.4) catalyse the dephosphorylation of diacylglycerol pyrophosphate (DGPP) and phosphatidic acid (PA), which are secondary messengers in abscisic acid (ABA) signalling. In this study, we investigated the effect of ABA on the expression of AtLPP genes as they encode putative ABA-signalling partners. We observed that AtLPP2 expression was down-regulated by ABA and we performed experiments on Atlpp2-2, an AtLPP2 knockout mutant, to determine whether AtLPP2 was involved in ABA signalling. We observed that Atlpp2-2 plantlets contained about twice as much PA as the wild-type Col-0 and exhibited higher PA kinase (PAK) activity than Col-0 plants. In addition, we showed that ABA stimulated diacylglycerol kinase (DGK) activity independently of AtLPP2 activity but that the ABA-stimulation of PAK activity recorded in Col-0 was dependent on AtLPP2. In order to evaluate the involvement of AtLPP2 activity in guard cell function, we measured the ABA sensitivity of Atlpp2-2 stomata. The inhibition of stomatal opening was less sensitive to ABA in Atlpp2-2 than in Col-0. Watered and water-stressed plants of the two genotypes accumulated ABA to the same extent, thus leading us to consider Atlpp2-2 an ABA-signalling mutant. Taken together our observations show that AtLPP2 is a part of ABA signalling and participate to the regulation of stomatal movements. |
| Databáze: | OpenAIRE |
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